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SJ000291942

Name: SJ000291942
Brand: TargetMol
SKU: T4662
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T4662Cas No. 425613-09-8

SJ000291942 is an activator of the canonical bone morphogenetic proteins (BMP) signaling pathway.

SJ000291942

🥰Excellent

Purity: 99.66%

Catalog No. T4662Cas No. 425613-09-8

SJ000291942 is an activator of the canonical bone morphogenetic proteins (BMP) signaling pathway.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$30	In Stock	In Stock
2 mg	$39	In Stock	In Stock
5 mg	$61	In Stock	In Stock
10 mg	$107	In Stock	In Stock
25 mg	$228	In Stock	In Stock
50 mg	$388	In Stock	In Stock
100 mg	$619	In Stock	In Stock
200 mg	$877	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$68	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.66%

Appearance:Solid

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description

SJ000291942 is an activator of the canonical bone morphogenetic proteins (BMP) signaling pathway.

In vitro

SJ000291942 is the most potent compound in causing severe ventralization in embryos, displaying higher mortality rates at lower doses compared to controls and two other compounds, indicating its strong induction of ventralization through enhanced BMP signaling activity. It elevates bmp2b and szl expression levels, suggesting activation of the canonical BMP signaling pathway in zebrafish assays. Further, immunoblotting experiments on C33A-2D2 cell lysates treated with SJ000291942 show its ability to activate SMAD1/5/8 phosphorylation within 1 hour in a serum-free medium, mirroring its high activity observed in zebrafish. Additionally, SJ000291942 significantly induces phosphorylation of Extracellular Signal-regulated Kinase, ERK1/2 (P-ERK1/2). High doses of BMP4 treatments (100 and 300ng) result in a gene expression profile similar to that of osteoblasts, while a low dose (10ng) of BMP4 closely matches the effects observed with 25μM of both compound 3 and SJ000291942 treatments.

Chemical Properties

Molecular Weight	318.3
Formula	C₁₆H₁₅FN₂O₄
Cas No.	425613-09-8
Smiles	CCc1ccc(OCC(=O)Nc2ccc(F)c(c2)[N+]([O-])=O)cc1
Relative Density.	1.327 g/cm3 (Predicted)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (188.5 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (6.28 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.1417 mL	15.7085 mL	31.4169 mL	157.0845 mL
5 mM	0.6283 mL	3.1417 mL	6.2834 mL	31.4169 mL
10 mM	0.3142 mL	1.5708 mL	3.1417 mL	15.7085 mL
20 mM	0.1571 mL	0.7854 mL	1.5708 mL	7.8542 mL
50 mM	0.0628 mL	0.3142 mL	0.6283 mL	3.1417 mL
100 mM	0.0314 mL	0.1571 mL	0.3142 mL	1.5708 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc