Scoulerine HCl is a multi-target inhibitor with antibacterial, antitumor, and antioxidant activities. Scoulerine HCl targets GABA receptors and BACE1 and inhibits mitochondrial dehydrogenase activity, thereby suppressing cancer cell proliferation, migration, invasion, epithelial-mesenchymal transition, and stemness maintenance. Scoulerine HCl also disrupts microtubule structure by acting on the PI3K/Akt/mTOR signaling axis and α1D-adrenergic receptors, thereby inducing cell cycle arrest and apoptosis. Additionally, Scoulerine HCl regulates endoplasmic reticulum stress and mitochondrial function, and possesses various pharmacological activities, including anti-Plasmodium falciparum and antiemetic and antitussive effects.

Methods: A549 (lung cancer), A2780 (ovarian cancer), SK-BR-3, and MCF-7 (breast cancer) cells were treated with scoulerin HCl (6451-73-6, free base) at concentrations of 1, 5, 10, 20, and 50 μM for 72 consecutive hours, and cell proliferation was monitored using real-time impedance analysis.
Results: Scoulerin HCl (6451-73-6 free base) exhibited concentration-dependent inhibition of adherent cancer cell proliferation, with significant inhibition observed at concentrations of 10 μM and above. [1]
Methods: Jurkat and MOLT-4 leukemia cells were treated with Scoulerine HCl (2.5, 5, 10, 15, 20 μM) for 24 and 48 hours, and live cells were counted using the trypan blue exclusion assay.
Results: Scoulerine HCl exhibited concentration- and time-dependent reductions in cell viability and proliferation. [1]
Methods: OVCAR3 cells were treated with Scoulerine HCl (5, 10 μmol/L) were added to OVCAR3 cells and pretreated for 48 hours; after treatment, the cells were cultured for an additional 10 days, and cell cloning ability was assessed via crystal violet staining and spectrophotometry.
Results: Scoulerine HCl exhibited a dose-dependent reduction in cell cloning ability, with an inhibition rate of approximately 78.3% at 10 μmol/L.[2]

Methods: To investigate the in vivo antitumor efficacy of Scoulerine HCl (6451-73-6, free base), BALB/c nude mice were used. SK-OV-3 cells were implanted subcutaneously, and administration of Scoulerine HCl (6451-73-6, free base) (1, 2.5, 5, 10 mg/kg) until the end of the experiment.
Results: Scoulerine HCl reduced tumor weight and volume in a dose-dependent manner; at 10 mg/kg, tumor weight decreased from approximately 2.1 g to approximately 0.7 g, and volume decreased from approximately 1800 mm³ to approximately 600 mm³. [2]