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Sal003

Name: Sal003
Brand: TargetMol
SKU: T2417
Price: 32 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T2417Cas No. 1164470-53-4

Sal003, an effective cell-permeable analog, inhibitis the eIF2α phosphatase.

Sal003

🥰Excellent

Purity: 99.77%

Catalog No. T2417Cas No. 1164470-53-4

Sal003, an effective cell-permeable analog, inhibitis the eIF2α phosphatase.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$32	In Stock	In Stock
2 mg	$45	In Stock	In Stock
5 mg	$67	In Stock	In Stock
10 mg	$105	In Stock	In Stock
25 mg	$192	In Stock	In Stock
50 mg	$285	In Stock	In Stock
100 mg	$423	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$68	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.77%

Color:White

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COA

Product Introduction

Bioactivity

Description	Sal003, an effective cell-permeable analog, inhibitis the eIF2α phosphatase.
In vitro	In mouse embryonic fibroblasts, Sal003 induced depolymerization of polysaccharides by increasing the phosphorylation of eIF2a. Sal003 increased eIF2a phosphorylation by blocking eIF2a phosphodiesterase activity, and Sal003 enhanced the apoptotic signaling pathway induced by cytotoxicity by induced phosphorylation of eIF2α.
In vivo	In mouse embryonic fibroblasts, Sal003 induced depolymerization of polysaccharides by increasing the phosphorylation of eIF2a. Sal003 increased eIF2a phosphorylation by blocking eIF2a phosphodiesterase activity, and Sal003 enhanced the apoptotic signaling pathway induced by cytotoxicity by induced phosphorylation of eIF2α.
Kinase Assay	Catalytic assay: MEK5 protein isolated from the baculovirus expression system is used to measure kinase activity utilizing PKLight ATP Detection Reagent. The assay is performed using 15 nM GST-MEK5 and 0.75 μM ATP in assay buffer consisting of 25 mM Hepes, pH 7.5, 10 mM MgCl2, 50 mM KCl, 0.2% BSA, 0.01% CHAPS, 100 μM Na3VO4, 0.5 mM DTT and 1% DMSO in the presence of varying concentrations of BIX02189. The kinase reaction mixture is incubated for 90 minutes at room temperature followed by addition of 10 μL of ATP detection reagent for 15 minutes. The relative light unit (RLU) signal is measured and the RLU signals are converted to percent of control (POC) values for the determination of IC50 value.

Chemical Properties

Molecular Weight	463.21
Formula	C₁₈H₁₅Cl₄N₃OS
Cas No.	1164470-53-4
Smiles	Clc1ccc(NC(=S)NC(NC(=O)\C=C\c2ccccc2)C(Cl)(Cl)Cl)cc1
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 46.3 mg/mL (99.95 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 2 mg/mL (4.32 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1588 mL	10.7942 mL	21.5885 mL	107.9424 mL
5 mM	0.4318 mL	2.1588 mL	4.3177 mL	21.5885 mL
10 mM	0.2159 mL	1.0794 mL	2.1588 mL	10.7942 mL
20 mM	0.1079 mL	0.5397 mL	1.0794 mL	5.3971 mL
50 mM	0.0432 mL	0.2159 mL	0.4318 mL	2.1588 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc