ACP5 Protein, Mouse, Recombinant (His)

Name: ACP5 Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-01414
Rating: 4.5 (1 reviews)

(Synonyms: TRAP, TRACP, acid phosphatase 5, tartrate resistant) Copy Product Info

Synonyms: TRAP, TRACP, acid phosphatase 5, tartrate resistant

Catalog No. TMPY-01414 Copy Product Info

ACP5 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 35.8 kDa and the accession number is Q05117.

Pack Size	Price	USA Stock	Global Stock
5 µg	$143	7-10 days	7-10 days
10 µg	$237	7-10 days	7-10 days
20 µg	$458	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:> 95 % as determined by SDS-PAGE

Appearance:Solution

Color:Transparent

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). The specific activity is >9,000 pmoles/min/μg.
Description	ACP5 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 35.8 kDa and the accession number is Q05117.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q05117
Construction	A DNA sequence encoding the pro form of mouse ACP5 (NP_031414.1) (Met 1-Pro 327) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Ala 23
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	TRAP, TRACP, acid phosphatase 5, tartrate resistant
Research Background	Tartrate-resistant acid phosphatase (TRACP) or acid phosphatase 5, tartrate resistant (ACP5 or TRAP) is a glycosylated monomeric metalloenzyme expressed in mammals. TRACP is associated with osteoblast migration to bone resorption sites, and, once there, TRACP is believed to initiate osteoblast differentiation, activation, and proliferation. TRACP once considered to be just a histochemical marker of osteoclasts is now recognised to be a molecule of widespread occurrence with functions in both the skeleton and the immune system. Two forms of TRACP circulate in human blood, TRACP 5a derived from macrophages and dendritic cells, and TRACP-5b derived from osteoclasts. Recent data have demonstrated the utility of TRACP-5b as a marker of osteoclast number and bone resorption, and serum TRACP-5a as a marker of inflammatory conditions. TRACP is expressed by osteoclasts, macrophages, dendritic cells and a number of other cell types. It has a critical role in many biological processes including skeletal development, collagen synthesis and degradation, the mineralisation of bone, cytokine production by macrophages and dendritic cells, macrophage recruitment, dendritic cell maturation and a role in the development of Th1 responses.

Chemical Properties

Molecular Weight

35.8 kDa (predicted); 35.8 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.