Shopping Cart

Your shopping cart is currently empty

RG108

Name: RG108
Brand: TargetMol
SKU: T2038
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

😃Good

Catalog No. T2038Cas No. 48208-26-0

Alias N-Phthalyl-L-tryptophan

RG108 (N-Phthalyl-L-tryptophan) is a DNA methyltransferase inhibitor with an IC50 of 115 nM.

RG108

Copy Product Info

😃Good

Purity: 99.43%

Catalog No. T2038Alias N-Phthalyl-L-tryptophanCas No. 48208-26-0

RG108 (N-Phthalyl-L-tryptophan) is a DNA methyltransferase inhibitor with an IC50 of 115 nM.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$39	In Stock	In Stock
10 mg	$63	In Stock	In Stock
25 mg	$119	In Stock	In Stock
50 mg	$216	In Stock	In Stock
100 mg	$302	In Stock	In Stock
200 mg	$423	In Stock	In Stock
500 mg	$687	Inquiry	Inquiry
1 mL x 10 mM (in DMSO)	$51	In Stock	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.43%

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	RG108 (N-Phthalyl-L-tryptophan) is a DNA methyltransferase inhibitor with an IC50 of 115 nM.
Targets&IC50	DNA methyltransferase:115 nM
In vivo	In iPS cells derived from SM, RG108 significantly reduces DNA activity. In tumor cells, RG108 inhibits gene demethylation and reactivation without affecting the methylation of telomeric gene sequences.
Kinase Assay	In vitro methylation assay: The substrate DNA for the in vitro methylation assay is a 798 bp fragment (?423/+375 relative to the initiation codon) from the promoter region of the human p16Ink4a gene. The methylation reaction contains 350 to 400 ng substrate DNA and 4 units of M.SssI methylase (0.5 μM) in a final volume of 50 μL. Inhibitors are added to final concentrations of 10, 100, 200, and 500 μM, respectively. Reactions are done at 37 °C for 2 hours. After completion, the reaction is inactivated at 65 °C for 15 minutes and the DNA is purified using PCR Purification kit. Three hundred nanograms of purified DNA is digested for 3 hours at 60 °C with 30 units of BstUI and analyzed on 2% Tris-borate EDTA agarose gels.
Cell Research	For the determination of cellular growth and viability, cells are stained with trypan blue and counted using a standard counting grid.(Only for Reference)
Synonyms	N-Phthalyl-L-tryptophan

Chemical Properties

Molecular Weight	334.33
Formula	C₁₉H₁₄N₂O₄
Cas No.	48208-26-0
Smiles	[C@H](CC=1C=2C(NC1)=CC=CC2)(C(O)=O)N3C(=O)C=4C(C3=O)=CC=CC4
Relative Density.	1.502 g/cm3 (Predicted)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 33.4 mg/mL (99.9 mM), Sonication is recommended.

DMSO: 255 mg/mL (762.72 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.98 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9911 mL	14.9553 mL	29.9106 mL	149.5528 mL
5 mM	0.5982 mL	2.9911 mL	5.9821 mL	29.9106 mL
10 mM	0.2991 mL	1.4955 mL	2.9911 mL	14.9553 mL
20 mM	0.1496 mL	0.7478 mL	1.4955 mL	7.4776 mL
50 mM	0.0598 mL	0.2991 mL	0.5982 mL	2.9911 mL

DMSO

	1mg	5mg	10mg	50mg
100 mM	0.0299 mL	0.1496 mL	0.2991 mL	1.4955 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc