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R-1479

Name: R-1479
Brand: TargetMol
SKU: TQ0162
Price: 112 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. TQ0162Cas No. 478182-28-4

Alias 4'-Azidocytidine

R-1479 (4'-Azidocytidine) is an RdRp inhibitor with an IC50 value of 1.28 μM for HCV RNA replication in the HCV subgenomic replicon system.

R-1479

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Purity: 99.95%

Catalog No. TQ0162Alias 4'-AzidocytidineCas No. 478182-28-4

R-1479 (4'-Azidocytidine) is an RdRp inhibitor with an IC50 value of 1.28 μM for HCV RNA replication in the HCV subgenomic replicon system.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$112	In Stock	In Stock
5 mg	$286	In Stock	In Stock
10 mg	$397	In Stock	In Stock
25 mg	$662	In Stock	In Stock
50 mg	$936	In Stock	In Stock
100 mg	$1,260	In Stock	In Stock
200 mg	$1,690	-	In Stock
1 mL x 10 mM (in DMSO)	$353	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.95%

Appearance:Solid

Color:White

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	R-1479 (4'-Azidocytidine) is an RdRp inhibitor with an IC50 value of 1.28 μM for HCV RNA replication in the HCV subgenomic replicon system.
Targets&IC50	HCV replication:1.28 μM
In vitro	R-1479 inhibits HCV RNA replication and reduces Renillaluciferase activity of proliferating replicon cells in a dose-dependent manner[1]. R-1479 inhibits RNA synthesis mediated by NS5B with an IC50 value of 320 nM and the RNA polymerase encoded by HCV[2].
Kinase Assay	The membrane-associated, native HCV replicase complex is isolated from 2209-23 HCV replicon cells and a derived cell line carrying HCV replicon RNA with an S282T mutation in the NS5B coding sequence. The in vitro replicase assay contain 10 μL of cytoplasmic membrane fraction, 50 mM HEPES (pH 7.5), 10 mM KCl, 10 mM dithiothreitol, 5 mM MgCl2, 20 μg/mL actinomycin D, 1 mM ATP, 1 mM GTP, 1 mM UTP, 30 μCi of [α-33P]CTP (3000 Ci/mmol, 10 mCi/mL), 1 unit/μL SUPERase•In, 10 mM creatine phosphate, and 200 μg/mL creatine phosphokinase in a final volume of 25 μL [1].
Synonyms	4'-Azidocytidine

Chemical Properties

Molecular Weight	284.23
Formula	C₉H₁₂N₆O₅
Cas No.	478182-28-4
Smiles	Nc1ccn([C@@H]2O[C@@](CO)(N=[N+]=[N-])[C@@H](O)[C@H]2O)c(=O)n1
Relative Density.	1.31g/cm3

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 90 mg/mL (316.64 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (11.61 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.5183 mL	17.5914 mL	35.1828 mL	175.9139 mL
5 mM	0.7037 mL	3.5183 mL	7.0366 mL	35.1828 mL
10 mM	0.3518 mL	1.7591 mL	3.5183 mL	17.5914 mL
20 mM	0.1759 mL	0.8796 mL	1.7591 mL	8.7957 mL
50 mM	0.0704 mL	0.3518 mL	0.7037 mL	3.5183 mL
100 mM	0.0352 mL	0.1759 mL	0.3518 mL	1.7591 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc