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Pitstop 2

Name: Pitstop 2
Brand: TargetMol
SKU: T26357
Price: 34 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T26357Cas No. 1419320-73-2

Pitstop2 is an amphipathic protein-bound inhibitor of the clathrin terminal domain (IC50=12 μM) with selectivity. Pitstop2 severely interferes with receptor-mediated endocytosis, HIV entry and synaptic vesicle cycling. Pitstop2 has antitumor activity.

Pitstop 2

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Purity: 99.54%

Catalog No. T26357Cas No. 1419320-73-2

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$34	In Stock	In Stock
5 mg	$80	In Stock	In Stock
10 mg	$139	In Stock	In Stock
25 mg	$289	In Stock	In Stock
50 mg	$455	In Stock	In Stock
100 mg	$672	-	In Stock
200 mg	$957	-	In Stock
1 mL x 10 mM (in DMSO)	$88	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.54%

Appearance:Solid

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description

In vitro

METHODS: HeLa cells were pre-incubated with Pitstop2 (10-30 µM) for 15 min, followed by incubation with Alexa568-Tf for 15 min, and transferrin (Tf) uptake was detected by live cell microscopy.
RESULTS: HeLa cells efficiently internalized Tf into perinuclear circulating endosomes when exposed to Alexa56-Tf. Preincubation of HeLa cells with Pitstop2 resulted in a dose-dependent inhibition of Tf uptake with IC50 values of 12-15 µM, which was very similar to that measured by in vitro blockade of the function of lattice protein TD. [1]
METHODS: Asynchronously growing HeLa cells were treated with Pitstop2 (0.001-100 µM) for 6 h and assayed for mitotic index.
RESULTS: Acute treatment of HeLa cells with Pitstop2 for 6 h resulted in a significant increase in mitotic index, suggesting that Pitstop2 activates the SAC. The effect of Pitstop2 on mitotic index was concentration-dependent, and significant at concentrations as low as 0.01 µM. [2]

Chemical Properties

Molecular Weight	473.36
Formula	C₂₀H₁₃BrN₂O₃S₂
Cas No.	1419320-73-2
Smiles	S(NC=1S/C(=C\C2=CC=C(Br)C=C2)/C(=O)N1)(=O)(=O)C=3C4=C(C=CC3)C=CC=C4
Relative Density.	1.60 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 22 mg/mL (46.48 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.23 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1126 mL	10.5628 mL	21.1256 mL	105.6279 mL
5 mM	0.4225 mL	2.1126 mL	4.2251 mL	21.1256 mL
10 mM	0.2113 mL	1.0563 mL	2.1126 mL	10.5628 mL
20 mM	0.1056 mL	0.5281 mL	1.0563 mL	5.2814 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc