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Pimonidazole

Name: Pimonidazole
Brand: TargetMol
SKU: T16537
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T16537Cas No. 70132-50-2

Pimonidazole accumulates in hypoxic cells via covalent binding with macromolecules or by forming reductive metabolites after the reduction of its nitro group. It can be used for qualitative and quantitative assessment of tumor hypoxia. Pimonidazole is a novel hypoxia marker for the complementary study of tumor hypoxia and cell proliferation in tumors.

Pimonidazole

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Purity: 99.47%

Catalog No. T16537Cas No. 70132-50-2

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$39	In Stock	In Stock
25 mg	$80	In Stock	In Stock
50 mg	$153	In Stock	In Stock
100 mg	$289	In Stock	In Stock
200 mg	$395	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.47%

Appearance:Solid

Color:Yellow

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COA HNMR LCMS

Product Introduction

Pimonidazole AI Summary

Pimonidazole exhibits multifaceted bioactivity, showing potential in both oncology and antiviral domains. It demonstrates aerobic growth inhibition in Chinese hamster cell line AA8 with an IC50 of 3030 µM and displays hypersensitivity in several cell lines such as UV4, EMT6, and FME. In addition, the compound is effective in reducing cell survival under hypoxic conditions as measured by the clonogenic assay, and it shows differential sensitivity under aerobic and hypoxic conditions with various CT10 values. For its physicochemical properties, Pimonidazole has a pKa of 8.8 in EMT6 mouse mammary tumor cells under different oxygen conditions and a partition coefficient of 0.4 in n-octanol at pH 7.4. The compound also exhibits broad-spectrum growth inhibitory effects against several human tumor cell lines, including those of breast, renal, non-small cell lung, leukemia, central nervous system, colon, melanoma, ovarian, and prostate origins, with a consistent GI50 of 100 µM. In the antiviral arena, Pimonidazole shows activity against SARS-CoV-2 by inhibiting cytotoxicity in Caco-2 cells with an inhibition rate of -10.89% at 10 µM after 48 hours. It also acts as an inhibitor of the SARS-CoV-2 3CL-Pro protease with inhibition rates of 41.54% at 20 µM, although its efficacy varies across different assays. Finally, Pimonidazole has displayed inhibitory activity against human HDAC6 enzyme, with an inhibition rate of 17.08% using a commercial peptide substrate and 9.72% with a custom peptide substrate..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description

Chemical Properties

Molecular Weight	254.29
Formula	C₁₁H₁₈N₄O₃
Cas No.	70132-50-2
Smiles	OC(CN1CCCCC1)Cn1ccnc1[N+]([O-])=O
Relative Density.	1.39g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (235.95 mM), Sonication is recommended.

H2O: 1 mg/mL (3.93 mM), Sonication is recommended.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.9325 mL	19.6626 mL	39.3252 mL	196.6259 mL

DMSO

	1mg	5mg	10mg	50mg
5 mM	0.7865 mL	3.9325 mL	7.8650 mL	39.3252 mL
10 mM	0.3933 mL	1.9663 mL	3.9325 mL	19.6626 mL
20 mM	0.1966 mL	0.9831 mL	1.9663 mL	9.8313 mL
50 mM	0.0787 mL	0.3933 mL	0.7865 mL	3.9325 mL
100 mM	0.0393 mL	0.1966 mL	0.3933 mL	1.9663 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc