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Pimonidazole hydrochloride

Name: Pimonidazole hydrochloride
Brand: TargetMol
SKU: T16536
Price: 41 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T16536Cas No. 70132-51-3

Pimonidazole hydrochloride accumulates in hypoxic cells via covalent binding with macromolecules or by forming reductive metabolites after the reduction of its nitro group. It can be used for qualitative and quantitative assessment of tumor hypoxia. Pimonidazole hydrochloride is a novel hypoxia marker for the complementary study of tumor hypoxia and cell proliferation in tumors.

Pimonidazole hydrochloride

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Purity: 99.5%

Catalog No. T16536Cas No. 70132-51-3

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$41	In Stock	In Stock
25 mg	$88	In Stock	In Stock
50 mg	$166	In Stock	In Stock
100 mg	$287	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.5%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	Pimonidazole hydrochloride accumulates in hypoxic cells via covalent binding with macromolecules or by forming reductive metabolites after the reduction of its nitro group. It can be used for qualitative and quantitative assessment of tumor hypoxia. Pimonidazole hydrochloride is a novel hypoxia marker for the complementary study of tumor hypoxia and cell proliferation in tumors.
In vitro	Pimonidazole, the exogenous hypoxia marker, is a 2-nitroimidazole compound, which forms covalent bonds with cellular macromolecules at oxygen levels below 1.3%[3].
In vivo	Pimonidazole is a 2-nitroimidazole that is reductively activated specifically in hypoxic cells and forms stable adducts with thiol groups in proteins, peptides, and amino acids. Additionally, the amount of pimonidazole that is detected is directly proportional to the level of hypoxia within tumors in vivo.

Chemical Properties

Molecular Weight	290.75
Formula	C₁₁H₁₉ClN₄O₃
Cas No.	70132-51-3
Smiles	Cl.OC(CN1CCCCC1)Cn1ccnc1[N+]([O-])=O
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 30 mg/mL (103.18 mM), Sonication is recommended.

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	3.4394 mL	17.1969 mL	34.3938 mL	171.9690 mL
5 mM	0.6879 mL	3.4394 mL	6.8788 mL	34.3938 mL
10 mM	0.3439 mL	1.7197 mL	3.4394 mL	17.1969 mL
20 mM	0.1720 mL	0.8598 mL	1.7197 mL	8.5985 mL
50 mM	0.0688 mL	0.3439 mL	0.6879 mL	3.4394 mL
100 mM	0.0344 mL	0.1720 mL	0.3439 mL	1.7197 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc