Shopping Cart

Your shopping cart is currently empty

N-Formyl-Met-Leu-Phe

Name: N-Formyl-Met-Leu-Phe
Brand: TargetMol
SKU: T7091
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

😃Good

Catalog No. T7091Cas No. 59880-97-6

Alias N-Formyl-MLF, fMLP

N-Formyl-Met-Leu-Phe (N-Formyl-MLF) (fMLF) is a synthetic peptide that acts as an agonist at formyl peptide receptors (FPR; Ki = 0.8 pM).

N-Formyl-Met-Leu-Phe

Copy Product Info

😃Good

Purity: 98.69%

Catalog No. T7091Alias N-Formyl-MLF, fMLPCas No. 59880-97-6

N-Formyl-Met-Leu-Phe (N-Formyl-MLF) (fMLF) is a synthetic peptide that acts as an agonist at formyl peptide receptors (FPR; Ki = 0.8 pM).

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$30	In Stock	In Stock
5 mg	$48	In Stock	In Stock
10 mg	$68	In Stock	In Stock
25 mg	$118	In Stock	In Stock
50 mg	$187	In Stock	In Stock
100 mg	$263	In Stock	In Stock
200 mg	$392	Inquiry	Inquiry

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:98.69%

Appearance:Solid

Color:White

Resource Download

COA HNMR MS HPLC

Product Introduction

Bioactivity

Description	N-Formyl-Met-Leu-Phe (N-Formyl-MLF) (fMLF) is a synthetic peptide that acts as an agonist at formyl peptide receptors (FPR; Ki = 0.8 pM).
In vitro	N-Formyl-Met-Leu-Phe receptor is associated with a Ni GTP regulatory protein, and N-Formyl-Met-Leu-Phe complex is important in the control of several neutrophil functions[1].
In vivo	N-Formyl-Met-Leu-Phe(fMLP) promoted bone formation in zebrafish and rabbits, suggesting its physiological relevance in vivo[2].
Animal Research	ebrafish (Danio rerio) embryos were collected from spontaneous spawnings reared at 28.5 ℃. Three days postfertilization (～1 day after hatching), feeding of the commercial powder for baby fish was started. Zebrafish embryos were stimulated with fMLP from 8 to 48 h postfertilization (hpf), washed, and raised at 28.5 ℃. At 5 days postfertilization (dpf), 20 zebrafish were stained with Alizarin Red for examination of the effect of fMLP on bone formation. Zebrafish were fixed in 4% paraformaldehyde for 12–18 h. Following fixation, fish were depigmented overnight in 1% KOH containing 3% H2O2 and immersed for 2 h in 0.5% KOH containing 0.05% Alizarin Red, pH 4.2. Alizarin-stained fish were then cleared again in 1% KOH and observed under a microscope[2].
Synonyms	N-Formyl-MLF, fMLP

Chemical Properties

Molecular Weight	437.55
Formula	C₂₁H₃₁N₃O₅S
Cas No.	59880-97-6
Smiles	CSCC[C@H](NC=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(O)=O
Relative Density.	1.198 g/cm3 (Predicted)
Sequence	Formyl-Met-Leu-Phe
Sequence Short	Formyl-MLF

Storage & Solubility Information

Storage

keep away from moisture | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 120 mg/mL (274.25 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (7.54 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2855 mL	11.4273 mL	22.8545 mL	114.2727 mL
5 mM	0.4571 mL	2.2855 mL	4.5709 mL	22.8545 mL
10 mM	0.2285 mL	1.1427 mL	2.2855 mL	11.4273 mL
20 mM	0.1143 mL	0.5714 mL	1.1427 mL	5.7136 mL
50 mM	0.0457 mL	0.2285 mL	0.4571 mL	2.2855 mL
100 mM	0.0229 mL	0.1143 mL	0.2285 mL	1.1427 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc