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N-Formyl-Met-Leu-Phe

Name: N-Formyl-Met-Leu-Phe
Brand: TargetMol
SKU: T7091
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T7091Cas No. 59880-97-6

Alias N-Formyl-MLF, fMLP

N-Formyl-Met-Leu-Phe (N-Formyl-MLF) (fMLF) is a synthetic peptide that acts as an agonist at formyl peptide receptors (FPR; Ki = 0.8 pM).

N-Formyl-Met-Leu-Phe

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Purity: 98.69%

Catalog No. T7091Alias N-Formyl-MLF, fMLPCas No. 59880-97-6

N-Formyl-Met-Leu-Phe (N-Formyl-MLF) (fMLF) is a synthetic peptide that acts as an agonist at formyl peptide receptors (FPR; Ki = 0.8 pM).

Pack Size	Price	Availability
2 mg	$30	In Stock
5 mg	$48	In Stock
10 mg	$68	In Stock
25 mg	$118	In Stock
50 mg	$187	In Stock
100 mg	$263	In Stock
200 mg	$392	In Stock

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Batch Information

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Purity:98.69%

Resource Download

COA HPLC MS HNMR

Product Introduction

Bioactivity

Description	N-Formyl-Met-Leu-Phe (N-Formyl-MLF) (fMLF) is a synthetic peptide that acts as an agonist at formyl peptide receptors (FPR; Ki = 0.8 pM).
In vitro	N-Formyl-Met-Leu-Phe receptor is associated with a Ni GTP regulatory protein, and N-Formyl-Met-Leu-Phe complex is important in the control of several neutrophil functions[1].
In vivo	N-Formyl-Met-Leu-Phe(fMLP) promoted bone formation in zebrafish and rabbits, suggesting its physiological relevance in vivo[2].
Animal Research	ebrafish (Danio rerio) embryos were collected from spontaneous spawnings reared at 28.5 ℃. Three days postfertilization (～1 day after hatching), feeding of the commercial powder for baby fish was started. Zebrafish embryos were stimulated with fMLP from 8 to 48 h postfertilization (hpf), washed, and raised at 28.5 ℃. At 5 days postfertilization (dpf), 20 zebrafish were stained with Alizarin Red for examination of the effect of fMLP on bone formation. Zebrafish were fixed in 4% paraformaldehyde for 12–18 h. Following fixation, fish were depigmented overnight in 1% KOH containing 3% H2O2 and immersed for 2 h in 0.5% KOH containing 0.05% Alizarin Red, pH 4.2. Alizarin-stained fish were then cleared again in 1% KOH and observed under a microscope[2].
Synonyms	N-Formyl-MLF, fMLP

Chemical Properties

Molecular Weight	437.55
Formula	C₂₁H₃₁N₃O₅S
Cas No.	59880-97-6
Smiles	CSCC[C@H](NC=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(O)=O
Relative Density.	1.198 g/cm3 (Predicted)
Sequence	Formyl-Met-Leu-Phe
Sequence Short	Formyl-MLF

Storage & Solubility Information

Storage

keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 82.5 mg/mL (188.55 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (7.54 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2855 mL	11.4273 mL	22.8545 mL	114.2727 mL
5 mM	0.4571 mL	2.2855 mL	4.5709 mL	22.8545 mL
10 mM	0.2285 mL	1.1427 mL	2.2855 mL	11.4273 mL
20 mM	0.1143 mL	0.5714 mL	1.1427 mL	5.7136 mL
50 mM	0.0457 mL	0.2285 mL	0.4571 mL	2.2855 mL
100 mM	0.0229 mL	0.1143 mL	0.2285 mL	1.1427 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc