Shopping Cart

Your shopping cart is currently empty

MY-1B

Name: MY-1B
Brand: TargetMol
SKU: T85335
Price: 129 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: MY1B) Copy Product Info

😃Good

Synonyms: MY1B

Catalog No. T85335 Copy Product Info

Purity: 99.81%

😃Good

MY-1B is a covalent inhibitor and an inhibitor of RNA methyltransferase NSUN2 (IC50 = 1.3 μM), featuring stereoselectivity, covalent binding to C271 of NSUN2, and the ability to target PSME1, disrupt proteasome regulatory complexes, and downregulate presentation of specific MHC-I subtypes.

Cas No. 2929308-79-0

Pack Size	Price	USA Stock	Global Stock
1 mg	$129	In Stock	In Stock
5 mg	$321	In Stock	In Stock
10 mg	$511	In Stock	In Stock
25 mg	$1,020	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:99.81%

ee:100%

Appearance:Solid

Color:White to Yellow

Resource Download

COA CSFC HNMR LCMS ORP

Product Introduction

Bioactivity

Description	MY-1B is a covalent inhibitor and an inhibitor of RNA methyltransferase NSUN2 (IC50 = 1.3 μM), featuring stereoselectivity, covalent binding to C271 of NSUN2, and the ability to target PSME1, disrupt proteasome regulatory complexes, and downregulate presentation of specific MHC-I subtypes.
Targets & IC50	NSUN2 methyltransferase:67 ± 15 M (Kinact/Ki), NSUN2:1.3 μM, NSUN2:2.4 µM (RNA methylation assays)
In vitro	Methods: The inhibitory effect of MY-1B (2.5–20 µM, 0–2000 seconds) on NSUN2 methyltransferase activity was determined by enzyme kinetic assays. Results: MY-1B inhibited NSUN2 activity with a Kinact/Ki value of 67 ± 15 M⁻¹.[1] Methods: In HEK293T cells, MY-1B (6.25–100 μM) was used to treat for 2 hours to assess binding to C1113 of DCAF1 protein. Results: MY-1B served as a stereo- and site-selective covalent ligand for C1113 of DCAF1.[2] Methods: In human prostate cancer cell lines, MY-1B (5 or 20 µM) was used to treat for 3 hours to assess binding to C271 of NSUN2 protein. Results: MY-1B bound to C271 of NSUN2 with stereoselectivity and site specificity.[3]
Synonyms	MY1B

Chemical Properties

Molecular Weight	436.3
Formula	C₂₂H₁₈BrN₃O₂
Cas No.	2929308-79-0
Smiles	C(NC=1C2=C(C=CC1)C=CC=N2)(=O)[C@H]3[C@H](CN3C(C=C)=O)C4=CC=C(Br)C=C4
Relative Density.	no data available

Storage & Solubility Information

Storage

Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (183.36 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (7.56 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2920 mL	11.4600 mL	22.9200 mL	114.6000 mL
5 mM	0.4584 mL	2.2920 mL	4.5840 mL	22.9200 mL
10 mM	0.2292 mL	1.1460 mL	2.2920 mL	11.4600 mL
20 mM	0.1146 mL	0.5730 mL	1.1460 mL	5.7300 mL
50 mM	0.0458 mL	0.2292 mL	0.4584 mL	2.2920 mL
100 mM	0.0229 mL	0.1146 mL	0.2292 mL	1.1460 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: MY-1B chemical structure | MY-1B in vitro | MY-1B formula | MY-1B molecular weight