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ML355

Name: ML355
Brand: TargetMol
SKU: T4347
Price: 38 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T4347Cas No. 1532593-30-8

ML355, a specific, effective 12-Lipoxygenase(12-LOX) inhibitors(IC50=0.34 μM), possess favorable ADME properties.

ML355

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🥰Excellent

Purity: 98.02%

Catalog No. T4347Cas No. 1532593-30-8

ML355, a specific, effective 12-Lipoxygenase(12-LOX) inhibitors(IC50=0.34 μM), possess favorable ADME properties.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$38	In Stock	In Stock
5 mg	$98	In Stock	In Stock
10 mg	$163	In Stock	In Stock
25 mg	$313	In Stock	In Stock
50 mg	$513	In Stock	In Stock
100 mg	$787	In Stock	In Stock
200 mg	$1,070	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$103	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:98.02%

Appearance:Solid

Color:White to Yellow

Resource Download

COA HPLC HNMR LCMS

Product Introduction

ML355 AI Summary

ML355 demonstrates a wide range of bioactivities. It has moderate oral bioavailability in CD-1 mice (F=19.8% at 30 mg/kg) and a relatively short half-life at 3 mg/kg via both oral and intravenous routes. It inhibits 12-LOX in mouse cells and human platelets with IC50 values of 1000.0 nM and 340.0 nM, respectively, and shows uncompetitive inhibition of N-terminal His6-tagged human platelet 12-LOX (Ki=350.0 nM) as well as competitive inhibition of this enzyme (Ki=720.0 nM). The compound is stable in PBS buffer, mouse plasma, and liver microsomes, and exhibits good solubility in PBS buffer at pH 7.4. It has weaker inhibitory activity against human 5-LOX and 15-LOX2 but selectively inhibits human reticulocyte 15-LOX1 over human platelet 12-LOX with an IC50 ratio of 29.0. In addition to its enzymatic inhibition properties, ML355 shows antiviral activity. It exhibits 6.99% inhibition of the SARS-CoV-2 3CL-Pro protease at 20 μM and inhibits SARS-CoV-2 induced cytotoxicity in VERO-6 cells at 10 μM following 48 hours of exposure to the virus. The compound affects various cell lines, impacting growth rates in HEK293T, U2OS, and human fibroblast cells with growth rates ranging from 0.18 to 1.0, indicating diverse effects on cell viability. In terms of protein stability, ML355 generally increases the thermal stability (melting temperature, TM) of different protein domains, although it decreases TM in one specific domain, signifying both stabilizing and destabilizing effects dependently on the targeted protein region..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	ML355, a specific, effective 12-Lipoxygenase(12-LOX) inhibitors(IC50=0.34 μM), possess favorable ADME properties.
Targets&IC50	12-LOX:0.34 μM

Chemical Properties

Molecular Weight	441.52
Formula	C₂₁H₁₉N₃O₄S₂
Cas No.	1532593-30-8
Smiles	COc1cccc(CNc2ccc(cc2)S(=O)(=O)Nc2nc3ccccc3s2)c1O
Relative Density.	1.481 g/cm3 (Predicted)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 125 mg/mL (283.11 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.53 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2649 mL	11.3245 mL	22.6490 mL	113.2452 mL
5 mM	0.4530 mL	2.2649 mL	4.5298 mL	22.6490 mL
10 mM	0.2265 mL	1.1325 mL	2.2649 mL	11.3245 mL
20 mM	0.1132 mL	0.5662 mL	1.1325 mL	5.6623 mL
50 mM	0.0453 mL	0.2265 mL	0.4530 mL	2.2649 mL
100 mM	0.0226 mL	0.1132 mL	0.2265 mL	1.1325 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc