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Lin28-let-7a antagonist 1

Name: Lin28-let-7a antagonist 1
Brand: TargetMol
SKU: T11851
Price: 93 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T11851Cas No. 2024548-03-4

Lin28-let-7a antagonist 1, with an IC50 of 4.03 μM for Lin28A-let-7a-1 interaction，and shows a clear antagonistic effect against the Lin28-let-7a interaction.

Lin28-let-7a antagonist 1

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Purity: 99.44%

Catalog No. T11851Cas No. 2024548-03-4

Lin28-let-7a antagonist 1, with an IC50 of 4.03 μM for Lin28A-let-7a-1 interaction，and shows a clear antagonistic effect against the Lin28-let-7a interaction.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$93	In Stock	In Stock
5 mg	$228	In Stock	In Stock
10 mg	$347	In Stock	In Stock
25 mg	$625	In Stock	In Stock
50 mg	$939	In Stock	In Stock
100 mg	$1,380	-	In Stock
1 mL x 10 mM (in DMSO)	$296	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.44%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	Lin28-let-7a antagonist 1, with an IC50 of 4.03 μM for Lin28A-let-7a-1 interaction，and shows a clear antagonistic effect against the Lin28-let-7a interaction.
Targets&IC50	Lin28A-let-7a-1:4.03 μM
In vitro	When the expression of both Lin28A and Lin28B are knocked-down using siRNA in JAR cells, the Lin28-let-7a antagonist 1-induced increase in cellular let-7 levels is clearly attenuated. Lin28A-let-7a-IN-1 increases mature let-7 levels in PA-1 cells that express high level of Lin28A. On the other hand, let-7 levels are not affected by 1 in MCF7 cells that rarely express Lin28 proteins. Taken together, Lin28-let-7a antagonist 1 specifically induces the increase in the cellular let-7 levels by targeting Lin28.Lin28-let-7a antagonist 1 shows a clear antagonistic effect against the Lin28A-let-7a-1 interaction with an IC50 of 4.03 μM. Interestingly, Lin28-let-7a antagonist 1 also inhibits the Lin28B-let-7 interaction, with slightly reduced potency. Dicer processing assay reveals that Lin28-let-7a antagonist 1 clearly blocks the formation of the Lin28A-pre-let-7g complex and induces miRNA processing, resulting in the generation of mature let-7g miRNA.

Chemical Properties

Molecular Weight	583.59
Formula	C₃₁H₂₉N₅O₇
Cas No.	2024548-03-4
Smiles	CC1(C)Oc2cc(N3CCN(CC3)C(=O)OCc3ccccc3)c(cc2-c2c1cnn2-c1ccc(cc1)C(O)=O)[N+]([O-])=O
Relative Density.	1.42 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (102.81 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.7135 mL	8.5677 mL	17.1353 mL	85.6766 mL
5 mM	0.3427 mL	1.7135 mL	3.4271 mL	17.1353 mL
10 mM	0.1714 mL	0.8568 mL	1.7135 mL	8.5677 mL
20 mM	0.0857 mL	0.4284 mL	0.8568 mL	4.2838 mL
50 mM	0.0343 mL	0.1714 mL	0.3427 mL	1.7135 mL
100 mM	0.0171 mL	0.0857 mL	0.1714 mL	0.8568 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc