This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
L-Buthionine-(S,R)-sulfoximine hydrochloride
Catalog No. T60407
L-Buthionine-(S,R)-sulfoximine hydrochloride is a potent, cell-permeable, fast-acting and irreversible inhibitor of G-glutamylcysteine synthetase (γ-GCS) and depletes cellular glutathione levels. L-Buthionine-(S,R)-sulfoximine has IC50s of 1.9 μM, 8.6 μM, and 29 μM in melanoma, breast and ovarian tumor specimens, respectively [1] [2].
All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
L-Buthionine-(S,R)-sulfoximine hydrochloride, CAS N/A
This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.
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Biological Description
Chemical Properties
Storage
& Solubility Information
Description
L-Buthionine-(S,R)-sulfoximine hydrochloride is a potent, cell-permeable, fast-acting and irreversible inhibitor of G-glutamylcysteine synthetase (γ-GCS) and depletes cellular glutathione levels. L-Buthionine-(S,R)-sulfoximine has IC50s of 1.9 μM, 8.6 μM, and 29 μM in melanoma, breast and ovarian tumor specimens, respectively [1] [2].
In vitro
L-Buthionine-(S,R)-sulfoximine synergistically enhanced BCNU activity against melanoma cell lines and human tumors. BSO (50 μM) treatment for 48 hr causes a 95% decrease in ZAZ and M14 melanoma cell line GSH levels, and a 60% decrease in GST enzyme activity. GST-μ protein and mRNA levels are significantly reduced in both cell lines. GST-π expression is unaffected. BSO enhancement of alkylator action may be related in part to down regulation of GST [1]. L-Buthionine-(S,R)-sulfoximine (BSO) induces oxidative stress in a cell by irreversibly inhibiting gamma-glutamylcysteine synthetase which is an essential enzyme for the synthesis of glutathione (GSH) [2]. L-Buthionine-(S,R)-sulfoximine (BSO) was demonstrated to induce ferroptosis in cancer cells [3].
In vivo
BSO treatment resulted in a significantly increased frequency of DNA deletions and decreased concentrations of GSH and cysteine. BSO treatment reduced GSH concentration in mouse fetuses by 27% and 55% at 2 mM and 20 mM BSO doses, respectively, compared with untreated mice. Co-treatment with 2 mM BSO and 20 mM NAC depleted GSH to a similar extent as 2 mM BSO, consistent with the function of BSO inhibiting the g-GCS enzyme indispensable for GSH synthesis [2].
Molecular Weight
258.77
Formula
C8H19ClN2O3S
Storage
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Dose Conversion
You can also refer to dose conversion for different animals.
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Method for preparing DMSO master liquid: mg
drug pre-dissolved in μL DMSO (Master liquid concentration
mg/mL),
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL PEG300, mix and clarify, next add μL
Tween 80,mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation:Take μL
DMSO master liquid, next add μL Corn oil,mix and clarify.
Note:
Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.