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K-252a

Name: K-252a
Brand: TargetMol
SKU: T15636
Price: 99 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T15636Cas No. 99533-80-9

Alias SF2370, Antibiotic SF 2370, Antibiotic K 252a

K-252a is a protein kinase inhibitor targeting serine-threonine, tyrosine, and CAM kinases. In vitro, it blocks NGF-induced TrkA signalling and neural differentiation, exhibiting antitumour, antibacterial, anti-inflammatory, and neuroprotective activities.

K-252a

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Purity: 98%

Catalog No. T15636Alias SF2370, Antibiotic SF 2370, Antibiotic K 252aCas No. 99533-80-9

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$99	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:98%

Color:White to Yellow

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Product Introduction

Bioactivity

Description	K-252a is a protein kinase inhibitor targeting serine-threonine, tyrosine, and CAM kinases. In vitro, it blocks NGF-induced TrkA signalling and neural differentiation, exhibiting antitumour, antibacterial, anti-inflammatory, and neuroprotective activities.
Targets&IC50	Ca2+/calmodulin-dependent kinase type II:270 nM, Phosphorylase kinase:1.7 nM, PKA:140 nM, PKC:470 nM
In vitro	Methods: LINC00641 overexpressing cell lines were treated with K-252a (1.7 nM, 6 hours), and the expression of target proteins in cells was detected by Western blotting. Results: K-252a can reduce the levels of p-Akt and p-TrkB in LINC00641 overexpressing cell lines. [3] Methods: PC12 subclone h cells were treated with K-252a (3-100 nM, 8 days) to study its effect on cell viability. Results: K-252a can inhibit NGF-induced neurite outgrowth. [4]
In vivo	Methods: Mice were treated with K-252a (20 mg/kg, intraperitoneal injection, once a day for 5 days) and their neurological function was evaluated. Results: K-252a was able to inhibit the TH-induced neuroprotective effect in mice. [5]
Synonyms	SF2370, Antibiotic SF 2370, Antibiotic K 252a

Chemical Properties

Molecular Weight	467.47
Formula	C₂₇H₂₁N₃O₅
Cas No.	99533-80-9
Smiles	C[C@]12N3C=4C5=C(C6=C(C4C=7C3=CC=CC7)CNC6=O)C=8C(N5[C@](O1)(C[C@@]2(C(OC)=O)O)[H])=CC=CC8
Relative Density.	1.67g/cm3

Storage & Solubility Information

Storage

store at low temperature | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (85.57 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.28 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1392 mL	10.6959 mL	21.3917 mL	106.9587 mL
5 mM	0.4278 mL	2.1392 mL	4.2783 mL	21.3917 mL
10 mM	0.2139 mL	1.0696 mL	2.1392 mL	10.6959 mL
20 mM	0.1070 mL	0.5348 mL	1.0696 mL	5.3479 mL
50 mM	0.0428 mL	0.2139 mL	0.4278 mL	2.1392 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc