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Indigo
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Catalog No. T2847Cas No. 482-89-3
Alias Indigotin
Indigo (Indigotin) is extracted from Indigo blue.
Indigo
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Purity: 99.01%
Catalog No. T2847Alias IndigotinCas No. 482-89-3
Indigo (Indigotin) is extracted from Indigo blue.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 1 g | $32 | In Stock | In Stock | |
| 2 g | $43 | In Stock | In Stock | |
| 5 g | $66 | - | In Stock | |
| 10 g | $95 | - | In Stock | |
| 1 mL x 10 mM (in DMSO) | $40 | In Stock | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Batch Information
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Purity:99.01%
Appearance:Solid
Color:Black
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Product Introduction
Indigo AI Summary
Indigo is a versatile bioactive molecule displaying various inhibitory and agonistic activities. It demonstrates notable bioactivity by inhibiting multiple enzymes and proteins, including CDK2 (IC50 of 70,000 nM), Src tyrosine kinase (IC50 of 28,000 nM), and beta-lactamase from Escherichia coli (IC50 of 30,000 nM, with a significant increase in inhibition upon incubation). It also shows an IC50 value of 1,000 nM against GST-tagged recombinant human CDK2/cyclin E expressed in insect SF-9 cells. Moreover, Indigo presents agonist activity toward the aryl hydrocarbon receptor (AhR) in human MCF7 cells and Saccharomyces cerevisiae YCM3 coexpressing ARNT, with EC50 values of 1,700 nM and 5.0 nM, respectively. Additionally, it inhibits [alpha-32P]ATP binding to DnaA protein from Escherichia coli (IC50 of 200,000 nM) and shows antimicrobial activity against Mycobacterium tuberculosis H37Rv in infected African green monkey Vero cells (MIC > 32,000 nM). Despite its range of effects, Indigo shows no significant inhibitory action in assays targeting Cyclin-dependent kinases 1 and 5, porcine GSK-3-alpha/beta kinase, or various bioactivities related to malate dehydrogenase and Chymotrypsinogen from Thermus flavus..
Note: Summary generated by AI. Data source: ChEMBL 
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Indigo (Indigotin) is extracted from Indigo blue. |
| Synonyms | Indigotin |
| Molecular Weight | 262.26 |
| Formula | C16H10N2O2 |
| Cas No. | 482-89-3 |
| Smiles | O=C1C(NC=2C1=CC=CC2)=C3C(=O)C=4C(N3)=CC=CC4 |
| Relative Density. | Ca. 1.35 g/cm3. Temperature:20 °C. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||
| Solubility Information | DMSO: 2.94 mg/mL (11.21 mM), Sonication is recommended.  | ||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||
DMSO
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Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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