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AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST)

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AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST)

Catalog No. TMPY-04451

AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619-1&Q9Y478-1&Q13131-1.

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Pack Size	Price	Availability
50 μg	$583	In Stock
100 μg	$994	7-10 days
200 μg	$1,690	7-10 days
500 μg	$3,420	7-10 days

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Biological Description

Biological Information	The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Description	AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619-1&Q9Y478-1&Q13131-1.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	His, GST
Accession Number	P54619-1&Q9Y478-1&Q13131-1
Construction	The Human PRKAG1 (P54619-1) (Met1-Pro331), constructed the plasmid 1; The Human PRKAB1 (Q9Y478-1) (Met1-Ile270), constructed the plasmid 2; The Human PRKAA1 (Q13131-1) (Met1-Gln559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purifie.
Protein Purity	> 85 % as determined by SDS-PAGE
Molecular Weight	160 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 7.5.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.

Dose Conversion

You can also refer to dose conversion for different animals. More

Tech Support

Please read the User Guide of Recombinant Proteins for more specific information.