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AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST)

Catalog No. TMPY-04451

AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus-Insect Cells

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST)
Pack Size Availability Price/USD Quantity
50 μg In stock $ 583.00
100 μg 5 days $ 994.00
200 μg 5 days $ 1,690.00
500 μg 5 days $ 3,420.00
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Biological Description
Technical Params
Product Properties
Biological Information The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Description AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus-Insect Cells
Species Human
Expression System Baculovirus-Insect Cells
Tag His,GST
Accession Number P54619-1&Q9Y478-1&Q13131-1
Construction A DNA sequence encoding the human PRKAG1 (P54619-1) (Met1-Pro331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478-1) (Met1-Ile270), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met1-Gln559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purifie.
Protein Purity > 85 % as determined by SDS-PAGE

Molecular Weight Approxiamtely 160 kDa
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Formulation Supplied as sterile 20mM Tris, 300mM NaCl, 10% Glycerol, pH 7.5. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.

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Keywords

recombinant recombinant-proteins proteins protein

 

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