AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST)

Catalog No. TMPY-04451

AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619-1&Q9Y478-1&Q13131-1.

AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST)

Catalog No. TMPY-04451

Pack Size	Price	Availability
50 μg	$583	In Stock
100 μg	$994	7-10 days
200 μg	$1,690	7-10 days
500 μg	$3,420	7-10 days

Bulk & Custom

Add to Cart

Questions

Select Batch

Resource Download

COA User Guide of Recombinant Proteins

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Information

Biological Activity	The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Description	AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619-1&Q9Y478-1&Q13131-1.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	His, GST
Accession Number	P54619-1&Q9Y478-1&Q13131-1
Construction	A DNA sequence encoding the human PRKAG1 (P54619-1) (Met1-Pro331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478-1) (Met1-Ile270), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met1-Gln559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purifie. Predicted N terminal: Met & Met & Met
Protein Purity	> 85 % as determined by SDS-PAGE
Molecular Weight	160 kDa (predicted); 38 (PRKAB1), 40 (PRKAG1) & 95 (PRKAA1) kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 7.5.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Shipping with blue ice.