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AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST)

Catalog No. TMPY-04451

AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619-1&Q9Y478-1&Q13131-1.

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AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST)
Pack Size Availability Price/USD Quantity
50 μg In stock $ 583.00
100 μg 5 days $ 994.00
200 μg 5 days $ 1,690.00
500 μg 5 days $ 3,420.00
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Biological Description
Technical Params
Product Properties
Biological Information The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Description AMPK (G1/B1/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619-1&Q9Y478-1&Q13131-1.
Species Human
Expression System Baculovirus Insect Cells
Tag His, GST
Accession Number P54619-1&Q9Y478-1&Q13131-1
Construction The Human PRKAG1 (P54619-1) (Met1-Pro331), constructed the plasmid 1; The Human PRKAB1 (Q9Y478-1) (Met1-Ile270), constructed the plasmid 2; The Human PRKAA1 (Q13131-1) (Met1-Gln559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purifie.
Protein Purity > 85 % as determined by SDS-PAGE

Molecular Weight 160 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Supplied as sterile 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 7.5.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.

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Keywords

recombinant recombinant-proteins proteins protein

 

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