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AMPK (G1/B2/A1) Heterotrimer Protein, Human, Recombinant (His & GST)
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Catalog No. TMPY-04452 Copy Product Info
AMPK (G1/B2/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619&O43741&Q13131-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $163 | - | In Stock | |
| 10 µg | $269 | 7-10 days | 7-10 days | |
| 20 µg | $453 | - | In Stock | |
| 50 µg | $903 | - | In Stock |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:90.6%
Appearance:Solution
Color:Transparent
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| Bioactivity | The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate. |
| Description | AMPK (G1/B2/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619&O43741&Q13131-1. |
| Species | Human |
| Expression System | Baculovirus Insect Cells |
| Tag | His, GST |
| Accession Number | P54619&O43741&Q13131-1 |
| Construction | A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified. Predicted N terminal: Met & Met & His |
| Protein Purity | > 90 % as determined by SDS-PAGE  |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Supplied as sterile 50 mM Tris, 200 mM NaCl, 2 mM GSH, 1 mM EDTA, 1 mM DTT, 10% gly, pH 7.4. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Molecular Weight | 160 kDa (predicted); 35, 37 & 95 kDa (reducing conditions) |
| Shipping | Proteins are shipped with blue ice. |
| Storage | It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: AMPK (G1/B2/A1) Heterotrimer Protein, Human, Recombinant (His & GST) chemical structure | AMPK (G1/B2/A1) Heterotrimer Protein, Human, Recombinant (His & GST) molecular weight
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