AMPK (G1/B2/A1) Heterotrimer Protein, Human, Recombinant (His & GST)

Catalog No. TMPY-04452

AMPK (G1/B2/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619&O43741&Q13131-1.

AMPK (G1/B2/A1) Heterotrimer Protein, Human, Recombinant (His & GST)

Catalog No. TMPY-04452

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50 μg	$904	In Stock

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Product Information

Biological Activity	The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Description	AMPK (G1/B2/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 160 kDa and the accession number is P54619&O43741&Q13131-1.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	His, GST
Accession Number	P54619&O43741&Q13131-1
Construction	A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified. Predicted N terminal: Met & Met & His
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	160 kDa (predicted); 35, 37 & 95 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Supplied as sterile 50 mM Tris, 200 mM NaCl, 2 mM GSH, 1 mM EDTA, 1 mM DTT, 10% gly, pH 7.4.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Shipping with blue ice.