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AMPK (G1/B2/A1) Heterotrimer Protein, Human, Recombinant (His & GST)

Catalog No. TMPY-04452

AMPK (G1/B2/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus-Insect Cells with His and GST tag. The predicted molecular weight is 160 kDa. Accession number: P54619&O43741&Q13131-1

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AMPK (G1/B2/A1) Heterotrimer Protein, Human, Recombinant (His & GST)
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50 μg In stock $ 904.00
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Biological Description
Technical Params
Product Properties
Biological Information The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Description AMPK (G1/B2/A1) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus-Insect Cells with His and GST tag. The predicted molecular weight is 160 kDa. Accession number: P54619&O43741&Q13131-1
Species Human
Expression System Baculovirus-Insect Cells
Tag His,GST
Accession Number P54619&O43741&Q13131-1
Construction A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Protein Purity > 90 % as determined by SDS-PAGE
Molecular Weight 160 kDa (predicted)
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Formulation Supplied as sterile 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% gly, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Stability & Storage

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.

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Keywords

recombinant recombinant-proteins proteins protein

 

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