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FLI-06

Name: FLI-06
Brand: TargetMol
SKU: T3075
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3075Cas No. 313967-18-9

Alias FLI 06

FLI-06 is a Notch signaling inhibitor (EC50: 2.3 μM).

FLI-06

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Purity: 99.86%

Catalog No. T3075Alias FLI 06Cas No. 313967-18-9

FLI-06 is a Notch signaling inhibitor (EC50: 2.3 μM).

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$30	In Stock	In Stock
2 mg	$44	In Stock	In Stock
5 mg	$68	In Stock	In Stock
10 mg	$101	In Stock	In Stock
25 mg	$181	In Stock	In Stock
50 mg	$328	In Stock	In Stock
100 mg	$571	In Stock	In Stock
500 mg	$1,250	-	In Stock
1 mL x 10 mM (in DMSO)	$75	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.86%

Appearance:Solid

Color:White to Yellow

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	FLI-06 is a Notch signaling inhibitor (EC50: 2.3 μM).
Targets&IC50	Notch:2.3 μM(EC50)
In vitro	In zebrafish embryos, FLI-06 at a concentration of 50µM inhibits the endogenous Notch signaling pathway.
In vivo	In kidney epithelial 293 cells, stable transfection of mutant APP results in significant amyloid deposition. FLI-06 alters the maturation pattern of the APP, subsequently terminating the release of APPs. Furthermore, in Hela cells overexpressing Notch green fluorescent protein, FLI-06 inhibits Notch transport and progression. FLI-06 disrupts the Golgi apparatus and inhibits the secretion of pre-transport proteins in the endoplasmic reticulum during the tubular to sheet-like morphology transition.
Kinase Assay	EC50 values of the test compounds are calculated from serial dilution series ranging from 200 to 0.1 μM. Cells are seeded in 96-well plates at a density of 5,000 cells per well in 100 μL medium. The next day, 100 μL medium containing each test compound is added. Cells are incubated for 16 h, fixed and processed for automated microscopy. EC50 estimates are calculated using four-parameter log-logistic fit with the package drc[1].
Synonyms	FLI 06

Chemical Properties

Molecular Weight	438.52
Formula	C₂₅H₃₀N₂O₅
Cas No.	313967-18-9
Smiles	CC1=C(C(C2=C(CC(C)(C)CC2=O)N1)c1ccc(cc1)[N+]([O-])=O)C(=O)OC1CCCCC1
Relative Density.	1.25 g/cm3 (Predicted)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 8.8 mg/mL (20.07 mM), Sonication is recommended.

DMSO: 60 mg/mL (136.82 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: 2.5 mg/mL (5.7 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2804 mL	11.4020 mL	22.8040 mL	114.0199 mL
5 mM	0.4561 mL	2.2804 mL	4.5608 mL	22.8040 mL
10 mM	0.2280 mL	1.1402 mL	2.2804 mL	11.4020 mL
20 mM	0.1140 mL	0.5701 mL	1.1402 mL	5.7010 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0456 mL	0.2280 mL	0.4561 mL	2.2804 mL
100 mM	0.0228 mL	0.1140 mL	0.2280 mL	1.1402 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc