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DMNB

Name: DMNB
Brand: TargetMol
SKU: T8637
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T8637Cas No. 20357-25-9

Alias 6-Nitroveratraldehyde

DMNB (6-Nitroveratraldehyde) is DNA-dependent protein kinase (DNA-PK) inhibitor with IC50 of 15 μM, an enzyme involved in the non-homologous end-joining (NHEJ) pathway of double-stranded DNA break (DSB) repair in human cells.

DMNB

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Purity: 97.87%

Catalog No. T8637Alias 6-NitroveratraldehydeCas No. 20357-25-9

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
200 mg	$29	-	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:97.87%

Appearance:Solid

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

DMNB AI Summary

DMNB demonstrates bioactivity as both an inhibitor and activator across various biological processes and enzyme assays. It inhibits human Jumonji Domain Containing 2E (JMJD2E), Tau Fibril Formation, Aldehyde Dehydrogenase 1 (ALDH1A1), 15-human lipoxygenase, Fructose-1,6-bisphosphate Aldolase, Hydroxyacyl-Coenzyme A Dehydrogenase, Type II, DNA-dependent protein kinase (DNA-PK), and Cytochrome P450 3A4, with potency values ranging from 177.8 nM to 39810.7 nM. Notably, it also serves as an activator of Cytochrome P450 3A4. In the context of antiviral activity, DMNB inhibits 5.75% of SARS-CoV-2 induced cytotoxicity in Caco-2 cells and shows slight inhibition of SARS-CoV-2 3CL-Pro protease by 0.8005% at 20 µM. However, it exhibited very minimal antiviral activity (-0.08%) against SARS-CoV-2 in VERO-6 cells under the same conditions. Furthermore, DMNB acts as a substrate for recombinant Haemophilus influenzae Chloramphenicol nitroreductase expressed in Escherichia coli, with an activity level of 132.0% under specific assay conditions involving NADPH. It also demonstrates varying inhibitory effects on human HDAC6, showing an 11.84% inhibition with a commercial peptide substrate but a decrease in activity (-2.7% inhibition) with a custom peptide substrate, highlighting the dependency on the assay's specific conditions..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	DMNB (6-Nitroveratraldehyde) is DNA-dependent protein kinase (DNA-PK) inhibitor with IC50 of 15 μM, an enzyme involved in the non-homologous end-joining (NHEJ) pathway of double-stranded DNA break (DSB) repair in human cells.
Synonyms	6-Nitroveratraldehyde

Chemical Properties

Molecular Weight	211.17
Formula	C₉H₉NO₅
Cas No.	20357-25-9
Smiles	COC1=C(OC)C=C(C(C=O)=C1)[N+]([O-])=O
Relative Density.	1.4194 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 22.5 mg/mL (106.55 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (9.47 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.7355 mL	23.6776 mL	47.3552 mL	236.7761 mL
5 mM	0.9471 mL	4.7355 mL	9.4710 mL	47.3552 mL
10 mM	0.4736 mL	2.3678 mL	4.7355 mL	23.6776 mL
20 mM	0.2368 mL	1.1839 mL	2.3678 mL	11.8388 mL
50 mM	0.0947 mL	0.4736 mL	0.9471 mL	4.7355 mL
100 mM	0.0474 mL	0.2368 mL	0.4736 mL	2.3678 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc