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Direct Blue 1
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Catalog No. T2194Cas No. 2610-05-1
Alias Pontamine sky blue, Chicago Sky Blue 6B
Direct Blue 1 (Pontamine sky blue), a counterstain for background autofluorescence in immunofluorescence histochemistry, structurally resembles glutamate and operates as a potent, competitive VGLUT inhibitor, not impacting plasma membrane transporters. This compound stands out as the inaugural small molecule PrP ligand to inhibit Aβ binding.
Direct Blue 1
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Purity: 97.79%
Catalog No. T2194Alias Pontamine sky blue, Chicago Sky Blue 6BCas No. 2610-05-1
Direct Blue 1 (Pontamine sky blue), a counterstain for background autofluorescence in immunofluorescence histochemistry, structurally resembles glutamate and operates as a potent, competitive VGLUT inhibitor, not impacting plasma membrane transporters. This compound stands out as the inaugural small molecule PrP ligand to inhibit Aβ binding.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 200 mg | $30 | In Stock | In Stock | |
| 500 mg | $42 | In Stock | In Stock | |
| 1 g | $58 | - | In Stock | |
| 2 g | $82 | - | In Stock | |
| 5 g | $133 | - | In Stock | |
| 1 mL x 10 mM (in DMSO) | $46 | In Stock | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Purity:97.79%
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Product Introduction
Direct Blue 1 AI Summary
Direct Blue 1 exhibits a range of biological activities across various targets. It effectively prevents HIV-1 induced T4 cell killing and replication with an EC50 of 4800.0 nM and has a similarity index (SI) value of 0.4. In Alzheimer's disease research, it inhibits synthetic amyloid beta-42 fibrillation by 1.1% as determined by ThT fluorescence and has an IC50 of 2580.0 nM for oligomerization inhibition measured via ELISA. For inflammatory response modulation, it reduces the allosteric activity of recombinant human MIF by more than 50.0% at 12 µg/ml and inhibits MIF's binding to the CD47 receptor with an IC50 of 810.0 nM. Furthermore, it demonstrates low efficacy in reducing SARS-CoV-2-infected Vero E6 cell viability, with an inhibition index of 0.0698. Additionally, Direct Blue 1 inhibits Pseudomonas aeruginosa IMPDH enzyme activity by 92.2% at 18.2 µM and with an IC50 of 1600.0 nM. It also targets human MIF by inhibiting various activities including binding to the CD74 receptor, tautomerase activity, and IL-8 secretion, with IC50 values between 370.0 nM and 3900.0 nM and a binding affinity Kd of 9040.0 nM..
Note: Summary generated by AI. Data source: ChEMBL 
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Direct Blue 1 (Pontamine sky blue), a counterstain for background autofluorescence in immunofluorescence histochemistry, structurally resembles glutamate and operates as a potent, competitive VGLUT inhibitor, not impacting plasma membrane transporters. This compound stands out as the inaugural small molecule PrP ligand to inhibit Aβ binding. |
| In vitro | Instructions I. Dissolve and prepare dye solution 1. Solution preparation: Dissolve Direct Blue 1 in water. Common dye concentrations range from 0.1% to 1% (w/v). The specific concentration is adjusted according to experimental needs. For example, when the dye solution concentration is 0.5%, 5 mg of dye is usually mixed with 1 mL of solvent. 2. Staining process: 1) Prepare samples: Prepare samples to be stained (such as textiles, proteins, cells, etc.) according to the type of experiment. 2) Staining solution: Add the prepared Direct Blue 1 solution to the sample. The staining time is usually 20 to 60 minutes, depending on the staining intensity requirements. 3) pH value: During staining, the pH value of the dye solution usually needs to be kept at acidic or neutral (pH 4-7). If the sample is sensitive to pH, precise pH control may be required. 4) Washing: After staining, wash with water or an appropriate buffer to remove unbound dye until the sample no longer releases dye. II. Applications 1. Protein or DNA staining: Direct Blue 1 can be used for staining in gel electrophoresis, especially for staining proteins and other biomacromolecules. 2. Cell staining: In cell biology experiments, Direct Blue 1 is often used for cell staining to facilitate observation of cell morphology or counting under a microscope. 3. Surface adsorption experiments: Because Direct Blue 1 can strongly adsorb to different surfaces (such as fibers, membranes or cell surfaces), it can also be used for surface adsorption or adsorption experiments. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Synonyms | Pontamine sky blue, Chicago Sky Blue 6B |
| Molecular Weight | 992.8 |
| Formula | C34H24N6Na4O16S4 |
| Cas No. | 2610-05-1 |
| Smiles | COc1c(ccc(c1)c1cc(c(cc1)/N=N/c1ccc2c(c1O)c(c(cc2S(=O)(=O)[O-])S(=O)(=O)[O-])N)OC)/N=N/c1ccc2c(c1O)c(c(cc2S(=O)(=O)[O-])S(=O)(=O)[O-])N.[Na+].[Na+].[Na+].[Na+] |
| Relative Density. | no data available |
| Storage | keep away from direct sunlight | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMSO: Slightly soluble  |
Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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