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BW-A 78U

Name: BW-A 78U
Brand: TargetMol
SKU: T5101
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T5101Cas No. 101155-02-6

Alias BW-A78U

BW-A 78U, a PDE4 inhibitor (IC50: 3 μM), does not inhibit lipopolysaccharide (LPS)-induced TNF-α release.

BW-A 78U

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Purity: 99.45%

Catalog No. T5101Alias BW-A78UCas No. 101155-02-6

BW-A 78U, a PDE4 inhibitor (IC50: 3 μM), does not inhibit lipopolysaccharide (LPS)-induced TNF-α release.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$33	In Stock	In Stock
5 mg	$103	In Stock	In Stock
10 mg	$155	In Stock	In Stock
25 mg	$262	In Stock	In Stock
50 mg	$377	In Stock	In Stock
100 mg	$533	In Stock	In Stock
200 mg	$732	-	In Stock
1 mL x 10 mM (in DMSO)	$37	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.45%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	BW-A 78U, a PDE4 inhibitor (IC50: 3 μM), does not inhibit lipopolysaccharide (LPS)-induced TNF-α release.
Targets&IC50	PDE4:3 μM
Cell Research	Mononuclear cells were incubated for 30 min with BW-A 78U (0.01-10 μM). The cells were then stimulated with lipopolysaccharide (10 μg/mL) overnight at 37°C in an atmosphere of 5% CO2 at 100% humidity. Cell-free supernatants were collected, centrifuged (2000 g), and stored frozen at -20°C before TNF-α determination. TNF-α concentrations in cell culture supernatants were determined by specific ELISA using a commercial kit. The sensitivity of the assay was 1 pg/mL. The absorbance at 450 nm was assessed with an ELISA reader.
Synonyms	BW-A78U

Chemical Properties

Molecular Weight	257.27
Formula	C₁₃H₁₂FN₅
Cas No.	101155-02-6
Smiles	CNc1ncnc2n(Cc3ccccc3F)cnc12
Relative Density.	1.36g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 45 mg/mL (174.91 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.77 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.8870 mL	19.4348 mL	38.8697 mL	194.3483 mL
5 mM	0.7774 mL	3.8870 mL	7.7739 mL	38.8697 mL
10 mM	0.3887 mL	1.9435 mL	3.8870 mL	19.4348 mL
20 mM	0.1943 mL	0.9717 mL	1.9435 mL	9.7174 mL
50 mM	0.0777 mL	0.3887 mL	0.7774 mL	3.8870 mL
100 mM	0.0389 mL	0.1943 mL	0.3887 mL	1.9435 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc