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Bobcat339

Name: Bobcat339
Brand: TargetMol
SKU: T5198
Price: 47 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T5198Cas No. 2280037-51-4

Bobcat339 is a novel cytosine-based TET enzyme inhibitor (IC50s: 33/73 uM for TET1/2), but not DNMT3a, does not inhibit the DNA methyltransferase DNMT3a.

Bobcat339

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Purity: 99.24%

Catalog No. T5198Cas No. 2280037-51-4

Bobcat339 is a novel cytosine-based TET enzyme inhibitor (IC50s: 33/73 uM for TET1/2), but not DNMT3a, does not inhibit the DNA methyltransferase DNMT3a.

Pack Size	Price	Availability
5 mg	$47	In Stock
10 mg	$76	In Stock
25 mg	$163	In Stock
50 mg	$263	In Stock
100 mg	$388	In Stock
1 mL x 10 mM (in DMSO)	$50	In Stock

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Purity:99.24%

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COA HPLC

Product Introduction

Bioactivity

Description	Bobcat339 is a novel cytosine-based TET enzyme inhibitor (IC50s: 33/73 uM for TET1/2), but not DNMT3a, does not inhibit the DNA methyltransferase DNMT3a.
Targets&IC50	TET1:33 μM (cell free), TET2:73 μM (cell free)
In vitro	HT-22 cells were treated with 10 μM Bobcat339 in 1% DMSO for 24 hrs and lysed. Cells treated with Bobcat339 had significantly reduced global 5hmC levels as compared to vehicle control.

Chemical Properties

Molecular Weight	297.74
Formula	C₁₆H₁₂ClN₃O
Cas No.	2280037-51-4
Smiles	Nc1nc(=O)n(cc1Cl)-c1cccc(c1)-c1ccccc1
Relative Density.	1.33 g/cm3 (Predicted)
Color	Cyan
Appearance	Solid

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 45 mg/mL (151.14 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (6.72 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.3586 mL	16.7932 mL	33.5864 mL	167.9318 mL
5 mM	0.6717 mL	3.3586 mL	6.7173 mL	33.5864 mL
10 mM	0.3359 mL	1.6793 mL	3.3586 mL	16.7932 mL
20 mM	0.1679 mL	0.8397 mL	1.6793 mL	8.3966 mL
50 mM	0.0672 mL	0.3359 mL	0.6717 mL	3.3586 mL
100 mM	0.0336 mL	0.1679 mL	0.3359 mL	1.6793 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc