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BCI

Name: BCI
Brand: TargetMol
SKU: T10486
Price: 48 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T10486Cas No. 1245792-51-1

Alias (E)-BCI

BCI ((E)-BCI) is an allosteric inhibitor of dual-specificity phosphatases (DUSP), with EC50 values of 13.3 μM for DUSP6 and 8.0 μM for DUSP1 in cells, and does not inhibit DUSP5.

BCI

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Purity: 99.66%

Catalog No. T10486Alias (E)-BCICas No. 1245792-51-1

BCI ((E)-BCI) is an allosteric inhibitor of dual-specificity phosphatases (DUSP), with EC50 values of 13.3 μM for DUSP6 and 8.0 μM for DUSP1 in cells, and does not inhibit DUSP5.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$48	In Stock	In Stock
5 mg	$120	In Stock	In Stock
10 mg	$197	In Stock	In Stock
25 mg	$313	In Stock	In Stock
50 mg	$472	In Stock	In Stock
100 mg	$666	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$89	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.66%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

BCI AI Summary

BCI exhibits a diverse range of bioactivities. It inhibits Myc-tagged human Dusp1 and Dusp6 in TPA-induced human HeLa cells, with IC50 values of 11500.0 nM and 12300.0 nM, respectively. The compound also inhibits Dusp6 basal phosphatase activity by 57.0% with a 3-o-methylfluoro-rescein phosphate substrate in the presence of ERK protein, and by 30.0% at a 10:1 enzyme to ERK protein ratio. In Tg(dusp:EGFP)pt6 zebrafish embryos, BCI enhances the expression of gata4 and nkx2.5 transcription factors with activity values of 81.0% and 91.0%, respectively, and increases the expression of cardiac hand2, myl7, and cmlc2 genes with activity values of 32.0%, 81.0%, and 81.0%, respectively. It also inhibits SARS-CoV-2 induced cytotoxicity in Caco-2 cells at 10 µM after 48 hours, showing an inhibition of -39.89%. While strongly inhibiting human recombinant Cdc25B and PTP1B with extremely low IC50 values, it shows much higher IC50 for human recombinant VHR. Although it fails to significantly inhibit SARS-CoV-2 3CL-Pro protease or virus-induced cytotoxicity in VERO-6 cells, BCI shows modest inhibition of human HDAC6 with an 8.83% activity in an enzymatic assay..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	BCI ((E)-BCI) is an allosteric inhibitor of dual-specificity phosphatases (DUSP), with EC50 values of 13.3 μM for DUSP6 and 8.0 μM for DUSP1 in cells, and does not inhibit DUSP5.
Synonyms	(E)-BCI

Chemical Properties

Molecular Weight	317.42
Formula	C₂₂H₂₃NO
Cas No.	1245792-51-1
Smiles	O=C1\C(=C\c2ccccc2)C(NC2CCCCC2)c2ccccc12
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (157.52 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 3.3 mg/mL (10.4 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.1504 mL	15.7520 mL	31.5040 mL	157.5200 mL
5 mM	0.6301 mL	3.1504 mL	6.3008 mL	31.5040 mL
10 mM	0.3150 mL	1.5752 mL	3.1504 mL	15.7520 mL
20 mM	0.1575 mL	0.7876 mL	1.5752 mL	7.8760 mL
50 mM	0.0630 mL	0.3150 mL	0.6301 mL	3.1504 mL
100 mM	0.0315 mL	0.1575 mL	0.3150 mL	1.5752 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc