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Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag)
(Synonyms: TNSALP, TNAP, MGC161443, IAP, HOPS, FLJ93059, FLJ40094, EC 3.1.3.1, AP-TNAP, APTNAP, ALPI, Akp2) Copy Product InfoSynonyms: TNSALP, TNAP, MGC161443, IAP, HOPS, FLJ93059, FLJ40094, EC 3.1.3.1, AP-TNAP, APTNAP, ALPI, Akp2
Catalog No. TMPK-00796 Copy Product Info
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 54.5 kDa and the accession number is P09923.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $68 | 7-10 days | 7-10 days | |
| 10 μg | $108 | 7-10 days | 7-10 days | |
| 20 μg | $177 | 7-10 days | 7-10 days | |
| 50 μg | $346 | 7-10 days | 7-10 days | |
| 100 μg | $581 | 7-10 days | 7-10 days | |
| 200 μg | $1,050 | 7-10 days | 7-10 days | |
| 500 μg | $2,330 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline phosphatase (Intestinal type) , His Tag with an affinity constant of 1.58 μM as determined in SPR assay. |
| Description | Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 54.5 kDa and the accession number is P09923. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | N-His-Flag |
| Accession Number | P09923 |
| Construction | Val20-Asp503 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | TNSALP, TNAP, MGC161443, IAP, HOPS, FLJ93059, FLJ40094, EC 3.1.3.1, AP-TNAP, APTNAP, ALPI, Akp2 |
| Research Background |
| Molecular Weight | 54.5 kDa (predicted). Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result. |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag) chemical structure | Alkaline phosphatase (Intestinal type) /ALPI Protein, Human, Recombinant (His & Flag) molecular weight
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