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AZD5582

Name: AZD5582
Brand: TargetMol
SKU: T14378
Price: 49 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T14378Cas No. 1258392-53-8

AZD5582 is an inhibitor of IAPs, which binds to the BIR3 domains cIAP1, cIAP2, and XIAP with IC50s of 15, 21, and 15 nM, respectively. It induces apoptosis.

AZD5582

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Purity: 98.75%

Catalog No. T14378Cas No. 1258392-53-8

AZD5582 is an inhibitor of IAPs, which binds to the BIR3 domains cIAP1, cIAP2, and XIAP with IC50s of 15, 21, and 15 nM, respectively. It induces apoptosis.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 mg	$49	In Stock	In Stock

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Batch Information

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Purity:98.75%

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	AZD5582 is an inhibitor of IAPs, which binds to the BIR3 domains cIAP1, cIAP2, and XIAP with IC50s of 15, 21, and 15 nM, respectively. It induces apoptosis.
Targets&IC50	CIAP2:21 nM (IC50), XIAP:15 nM (IC50), CIAP1:15 nM (IC50)
In vitro	AZD5582 (20 nM; 48 hours) inhibited cell viability by cooperating with IFNγ or viral double-stranded RNA (dsRNA) in H1975 NSCLC cells[2]. In HCC827 NSCLC cells, AZD5582 (20 nM; 48 hours) induced apoptosis by activating cell death and caspase-3/-8 through co-treatment with IFNγ[2]. Additionally, AZD5582 (20 nM; 17 or 25 hours) down-regulated cIAP-1, activated RIPK1 (an upstream regulator of caspase-8), and initiated both extrinsic (caspase-8) and intrinsic (caspase-9) apoptosis pathways, leading to the cleavage of caspase-3 and caspase-7[2].
In vivo	AZD5582 (intravenous injection; 0.1-3.0 mg/kg; once a week; 2 weeks) induces cIAP1 degradation and caspase 3 cleavage in tumor cells, leading to significant tumor resolution after two weeks. A medium dose (0.5 mg/kg) of AZD5582 degrades cIAP1 but requires additional time to achieve apoptosis-inducing effects[1].

Chemical Properties

Molecular Weight	1015.29
Formula	C₅₈H₇₈N₈O₈
Cas No.	1258392-53-8
Smiles	OC(=O)C(F)(F)F.CN[C@@H](C)C(=O)N[C@@H](C1CCCCC1)C(=O)N1CCC[C@H]1C(=O)N[C@@H]1[C@@H](Cc2ccccc12)OCC#CC#CCO[C@@H]1Cc2ccccc2[C@@H]1NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H](C)NC)C1CCCCC1
Relative Density.	1.26 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 66.25 mg/mL (65.25 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (1.97 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	0.9849 mL	4.9247 mL	9.8494 mL	49.2470 mL
5 mM	0.1970 mL	0.9849 mL	1.9699 mL	9.8494 mL
10 mM	0.0985 mL	0.4925 mL	0.9849 mL	4.9247 mL
20 mM	0.0492 mL	0.2462 mL	0.4925 mL	2.4624 mL
50 mM	0.0197 mL	0.0985 mL	0.1970 mL	0.9849 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc