Shopping Cart

Your shopping cart is currently empty

Apafant

Name: Apafant
Brand: TargetMol
SKU: T14300
Price: 466 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

😃Good

Catalog No. T14300Cas No. 105219-56-5

Alias WEB-2086, WEB2086, WEB 2086

Apafant (WEB 2086) is a highly potent platelet activating factor (PAF) antagonist that blocks eosinophil activation and can be used to study experimental allergic conjunctivitis in guinea pigs.

Apafant

Copy Product Info

😃Good

Purity: 99.56%

Catalog No. T14300Alias WEB-2086, WEB2086, WEB 2086Cas No. 105219-56-5

Apafant (WEB 2086) is a highly potent platelet activating factor (PAF) antagonist that blocks eosinophil activation and can be used to study experimental allergic conjunctivitis in guinea pigs.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$56	-	In Stock
5 mg	$147	-	In Stock
10 mg	$236	-	In Stock
25 mg	$466	-	In Stock
50 mg	Preferential	-	In Stock
1 mL x 10 mM (in DMSO)	$148	-	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.56%

Appearance:Solid

Color:White

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	Apafant (WEB 2086) is a highly potent platelet activating factor (PAF) antagonist that blocks eosinophil activation and can be used to study experimental allergic conjunctivitis in guinea pigs.
Targets&IC50	PAF:（ki）9.9 nM
In vivo	Guinea pigs were actively sensitized and OA solution was instilled into the eyes of guinea pigs on Days 15 and 17 to elicit the allergic conjunctivitis. To examine the effects of Apafant on allergic conjunctivitis, Apafant ophthalmic solution was topically administered 0.1%, 1%, q.i.d. through Days 14 to 17. Compared to the controls (vehicle treatments), the animals treated with Apafant ophthalmic solution showed significantly reduced clinical symptoms (redness and edema) and itch-scratch responses in both the first and second antigen challenges. [1]
Synonyms	WEB-2086, WEB2086, WEB 2086

Chemical Properties

Molecular Weight	455.96
Formula	C₂₂H₂₂ClN₅O₂S
Cas No.	105219-56-5
Smiles	O=C(N1CCOCC1)CCC=2SC3=C(C2)C(=NCC4=NN=C(N43)C)C=5C=CC=CC5Cl
Relative Density.	1.48g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 30 mg/mL (65.8 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (7.24 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1932 mL	10.9659 mL	21.9317 mL	109.6587 mL
5 mM	0.4386 mL	2.1932 mL	4.3863 mL	21.9317 mL
10 mM	0.2193 mL	1.0966 mL	2.1932 mL	10.9659 mL
20 mM	0.1097 mL	0.5483 mL	1.0966 mL	5.4829 mL
50 mM	0.0439 mL	0.2193 mL	0.4386 mL	2.1932 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc