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Anti-PAFR/PAF Receptor Polyclonal Antibody

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Catalog No. TMAB-01326

Anti-PAFR/PAF Receptor Polyclonal Antibody is a Rabbit antibody targeting PAFR/PAF Receptor. Anti-PAFR/PAF Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-PAFR/PAF Receptor Polyclonal Antibody

Anti-PAFR/PAF Receptor Polyclonal Antibody

😃Good
Catalog No. TMAB-01326
Anti-PAFR/PAF Receptor Polyclonal Antibody is a Rabbit antibody targeting PAFR/PAF Receptor. Anti-PAFR/PAF Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$372 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-PAFR/PAF Receptor Polyclonal Antibody is a Rabbit antibody targeting PAFR/PAF Receptor. Anti-PAFR/PAF Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Rabbit)
Verified Activity
1. Sample: LympH(Mouse) Lysate at 30 μg
Primary: Anti-RAFR/PAF (TMAB-01326) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
2. Blank control: 293T cells (blue). Primary Antibody: Rabbit Anti-PAFR/PAF antibody (TMAB-01326), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-01326, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
3. Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PTAFR Polyclonal Antibody, Unconjugated (TMAB-01326) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PTAFR Polyclonal Antibody, Unconjugated (TMAB-01326) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Sample:
Lane1: Lung (Rat) Lysate at 30 μg
Lane2: Brain (Rat) Lysate at 30 μg
Primary: Anti-PTAFR (TMAB-01326) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000 dilution;
Predicted band size: 38 kDa
Observed band size: 38 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1µg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe PAF receptor binds platelet-activating factor (PAF) and is thought to mediate its action via a G protein that activates a phosphatidylinositol-calcium second messenger system. PAF is a chemotactic phospholipid mediator that possesses potent inflammatory, smooth-muscle contractile and hypotensive activity. It has been implicated as a mediator in diverse pathologic processes, such as allergy, asthma, septic shock, arterial thrombosis, and inflammatory processes. The PAF receptor is induced by granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin-5 and n-butyrate. A diverse range of compounds act as PAF receptor antagonists; these may be useful pharmacologically.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PAFR
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Blood,Platelets,Macrophage / Inflamm.,GPCR
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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