Shopping Cart

Your shopping cart is currently empty

ANI-7

Name: ANI-7
Brand: TargetMol
SKU: T10325
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

😃Good

Catalog No. T10325Cas No. 931417-26-4

ANI-7 is an activator of the aryl hydrocarbon receptor (AhR) pathway, inhibiting the growth of various cancer cells and selectively inhibiting the growth of MCF-7 breast cancer cells (GI50: 0.56 μM).

ANI-7

Copy Product Info

😃Good

Purity: 99.07%

Catalog No. T10325Cas No. 931417-26-4

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$34	In Stock	In Stock
10 mg	$59	In Stock	In Stock
25 mg	$113	In Stock	In Stock
50 mg	$176	In Stock	In Stock
100 mg	$247	In Stock	In Stock
200 mg	$367	-	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products.If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.07%

Appearance:Solid

Color:Yellow

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description

In vitro

ANI-7 (2.5 μM; 24 hours; MCF10A and MDA-MB-468 cells) treatment induces significant S-phase and G2 + M-phase cell cycle arrest within 24 hours in MDA-MB-468 cells. ANI-7 (2 μM; 12-24 hours; MDA-MB-468 cells) significantly increases CHK2 content and phosphorylation and induces a significant increase in H2AXɣ, indicating DNA double-strand damage. GI50 values show ANI-7 has a GI50 of 0.38 μM in MCF-7 cells, while values of 3.0-42 μM are seen in lung, colon, ovary, neuronal, glial, prostate, and pancreas cell lines. The A431 vulva cell line shows appreciable growth inhibition (GI50 of 0.51 μM) by ANI-7. ANI-7 potently inhibits T47D, ZR-75-1, MCF-7, SKBR3, and MDA-MB-468 breast cancer cells (GI50 range 0.16-0.38 μM), moderately inhibits BT20 and BT474 cells (GI50 range 1-2 μM), and essentially fails to inhibit MDA-MB-231 and MCF10A cells (GI50 range 17-26 μM). Additionally, ANI-7 maintains its inhibitory effect on drug-resistant cells (MCF-7/VP16: GI50 of 0.21 μM) [1].

Chemical Properties

Molecular Weight	263.12
Formula	C₁₃H₈Cl₂N₂
Cas No.	931417-26-4
Smiles	Clc1ccc(cc1Cl)C(=C\c1ccc[nH]1)\C#N
Relative Density.	1.398 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 45 mg/mL (171.02 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.6 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.8005 mL	19.0027 mL	38.0055 mL	190.0274 mL
5 mM	0.7601 mL	3.8005 mL	7.6011 mL	38.0055 mL
10 mM	0.3801 mL	1.9003 mL	3.8005 mL	19.0027 mL
20 mM	0.1900 mL	0.9501 mL	1.9003 mL	9.5014 mL
50 mM	0.0760 mL	0.3801 mL	0.7601 mL	3.8005 mL
100 mM	0.0380 mL	0.1900 mL	0.3801 mL	1.9003 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc