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Vadimezan

Catalog No. T6273 CAS 117570-53-3

Synonyms: 伐地美生, NSC 640488, 5,6-Dimethylxanthenone-4-acetic Acid, ASA-404, DMXAA

Vadimezan is a fused tricyclic analogue of flavone acetic acid with potential antineoplastic activity. Vadimezan induces the cytokines tumor necrosis alpha (TNF-alpha), serotonin and nitric oxide, resulting in hemorrhagic necrosis and a decrease in angiogenesis. This agent also stimulates the anti-tumor activity of tumor-associated macrophages.

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Vadimezan, CAS 117570-53-3

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Vadimezan is a fused tricyclic analogue of flavone acetic acid with potential antineoplastic activity. Vadimezan induces the cytokines tumor necrosis alpha (TNF-alpha), serotonin and nitric oxide, resulting in hemorrhagic necrosis and a decrease in angiogenesis. This agent also stimulates the anti-tumor activity of tumor-associated macrophages.
Targets&IC50	DT-diaphorase:20 μM(Ki)
In vitro	In DLD-1 human colon carcinoma cells, DMXAA inhibits DT-diaphorase activity without significant effects on the activity of cytochrome b5 reductase and cytochrome P450 reductase. Combination of menadione and DMXAA leads to an increase in the antiproliferative activity of DLD-1 cells. [1] DMXAA, as an antiviral agent, inhibits VSV-induced cytotoxicity and influenza virus replication in RAW 264.7 macrophages. [2] A recent study shows that DMXAA has non-immune-mediated inhibitory effects against several kinase members of VEGFR (vascular endothelial growth factor receptor), such as VEGFR2 signalling in human umbilical vein endothelial cells. [3]
In vivo	DMXAA treatment significantly protects C57BL/6J mice infected i.n. with 200 p.f.u. mouse-adapted H1N1 influenza PR8 virus with 60% survival, while the control group only exhibited 20% survival. [2] DMXAA significantly delays tumor growth induced by chemical carcinogen, increases the time to tumor doubling and increases time from treatment to euthanasia. After the treatment of DMXAA, median tumor doubling time, median tumour tripling time and median time from treatment to euthanasia in tumor-bearing animals are increased by approximately 4.4-, 1.8- and 2.7-fold, respectively. [4]
Kinase Assay	DT-diaphorase activity and kinetic analysis of enzyme inhibition : Purified DT-diaphorase enzyme activity is assayed by measuring the reduction of cytochrome c at 550 nm on a Beckman DU 650 spectrophotometer. Each assay contains cytochrome c (70 μM), NADH (variable concentrations), purified DT-diaphorase (0.032 μg), and menadione (variable concentrations) in a final volume of 1 mL Tris–HCl buffer (50 mM, pH 7.4) containing 0.14% BSA. The reaction is started by the addition of NADH. Rates of reduction are calculated over the initial part of the reaction curve (30 seconds), and results are expressed in terms of μmol cytochrome c reduced/min/mg protein using a molar extinction coefficient of 21.1 mM?1 cm?1 for reduced cytochrome c. Enzyme assays are carried out at room temperature and all reactions are performed in triplicate. Inhibition of purified DT-diaphorase activity is performed by the inclusion of DMXAA (at various concentrations) in the reaction, and inhibition characteristics are determined by varying the concentration of NADH (constant menadione) or menadione (constant NADH) at several concentrations of inhibitor. Ki values are obtained by plotting 1/V against. The activity of DT-diaphorase in DLD-1 cells is determined by measuring the dicumarol-sensitive reduction of DCPIP at 600 nm. Briefly, DLD-1 cells in mid-exponential growth are harvested by scraping into ice-cold buffer (Tris–HCl, 25 mM, pH 7.4 and 250 mM sucrose) and sonicated on ice. Enzyme assay conditions are 2 mM NADH, 40 μM DCPIP, 20 μL of dicumarol (when required) in a final volume of 1 mL Tris–HCl (25 mM, pH 7.4) containing BSA (0.7 mg/mL). Results are expressed as the dicumarol-sensitive reduction of DCPIP using a molar extinction coefficient of 21 mM?1 cm?1. Protein levels are determined using the Bradford assay
Cell Research	DLD-1 human colon carcinoma and H460 human non-small cell lung carcinoma cells are routinely maintained as monolayer cultures in RPMI 1640 culture medium supplemented with foetal calf serum (10%), sodium pyruvate (2 mM), penicillin/streptomycin (50 IU mL?1/50 μg mL-1) and l-glutamine (2 mM). Chemosensitivity is assessed using the MTT assay and all assays are performed under aerobic conditions. Briefly, cells are plated into each well of a 96-well culture plate and incubated overnight in an atmosphere containing 5% CO2. Culture medium is completely removed from each well and replaced with medium containing a range of drug concentrations. In the case of menadione alone, the duration of drug exposure is 1 hour, after which the cells are washed twice with Hanks' balanced salt solution prior to the addition of 200 μL fresh RPMI 1640 medium to each well of the plate. In the case of DMXAA alone, the duration of drug exposure is 3 hours. Following a four-day incubation, cell survival is determined using the MTT assay. For combinations of DMXAA with menadione, cells are initially set up and a non-toxic (>95% cell survival) concentration of DMXAA is selected. Cells are then initially exposed to DMXAA (2 mM) for a period of 2 hours, following which the medium is removed and replaced with medium containing the inhibitor (DMXAA at a constant concentration of 2 mM) and menadione (at a range of drug concentrations). Following a further 7-hour incubation, cells are washed twice with Hanks' balanced salt solution prior to the addition of growth medium.(Only for Reference)

Synonyms	伐地美生, NSC 640488, 5,6-Dimethylxanthenone-4-acetic Acid, ASA-404, DMXAA
Molecular Weight	282.295
Formula	C17H14O4
CAS No.	117570-53-3

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 5.7 mg/mL (20 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Phillips RM. Biochem Pharmacol. 1999, 58(2), 303-310. 2. Shirey KA, et al. J Leukoc Biol. 2011, 89(3), 351-357. 3. Buchanan CM, et al. Clin Sci (Lond). 2012, 122(10), 449-457. 4. Liu JJ, et al. Cancer Chemother Pharmacol. 2007, 59(5), 661-669. 5. Chen C, Yan W, Zhang Y, et al. ANP Attenuates Intestinal Inflammation by Regulating STING Pathway[J]. Available at SSRN 3756807. 6. Zhao M, Wang B, Zhang C, et al. The DJ1-Nrf2-STING axis mediates the neuroprotective effects of Withaferin A in Parkinson’s disease[J]. Cell Death & Differentiation. 2021: 1-19.

Citations

1. Zhao M, Wang B, Zhang C, et al. The DJ1-Nrf2-STING axis mediates the neuroprotective effects of Withaferin A in Parkinson’s disease. Cell Death & Differentiation. 2021: 1-19. 2. Chen C, Zhang Y, Tao M, et al. Atrial Natriuretic Peptide Attenuates Colitis via Inhibition of the cGAS-STING Pathway in Colonic Epithelial Cells. International Journal of Biological Sciences. 2022, 18(4): 1737-1754.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Autophagy Compound Library Anti-Lung Cancer Compound Library Inhibitor Library Immunology/Inflammation Compound Library Drug Repurposing Compound Library Anti-Cancer Active Compound Library Apoptosis Compound Library Angiogenesis related Compound Library Anti-Cancer Drug Library

Related Products

Related compounds with same targets

Alpinone 3-acetate CCCP Vadimezan Cridanimod AX-024 hydrochloride Ginsenoside F3 RO8191 Deucravacitinib

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Keywords

Vadimezan 117570-53-3 免疫与炎症微生物学血管生成 IFNAR Influenza Virus STING VDA Interferon-α/β receptor ASA-404 Stimulator of Interferon Genes MITA TMEM173 NSC 640488 inhibit Inhibitor 5,6-Dimethylxanthenone-4-acetic Acid ASA404 ASA 404 DMXAA MPYS ERIS Interferon-alpha/beta receptor inhibitor

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