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VBIT-4

Name: VBIT-4
Brand: TargetMol
SKU: T13287
Price: 68 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T13287Cas No. 2086257-77-2

VBIT-4 is a voltage-dependent anion channel 1 (VDAC1) oligomerization inhibitor (Kd: 17 μM). VBIT-4 can be used for therapeutic purposes in apoptosis-associated disorders (such as neurodegenerative and cardiovascular diseases).

VBIT-4

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Purity: 99.93%

Catalog No. T13287Cas No. 2086257-77-2

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$68	In Stock	In Stock
5 mg	$163	In Stock	In Stock
10 mg	$289	In Stock	In Stock
25 mg	$496	In Stock	In Stock
50 mg	$828	In Stock	In Stock
100 mg	$1,090	-	In Stock
200 mg	$1,480	-	In Stock
1 mL x 10 mM (in DMSO)	$179	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.93%

Color:White to Yellow

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	VBIT-4 is a voltage-dependent anion channel 1 (VDAC1) oligomerization inhibitor (Kd: 17 μM). VBIT-4 can be used for therapeutic purposes in apoptosis-associated disorders (such as neurodegenerative and cardiovascular diseases).
Targets&IC50	VDAC1:17 Μm (kd), HEK293 cells:2.9 µM, VDAC1:1.9 ± 0.08 μM, Cytochrome c:1.8 ± 0.24 μM
In vitro	VBIT-4 targets the mitochondrial protein VDAC1, inhibits apoptosis and defends against mitochondrial dysfunction. VBIT-4 (0.1-10 μM) inhibits VDAC1 oligomerization, Cyto c release from mitochondria and apoptosis (in HEK-293 cells , IC50s of 1.9±0.08 μM, 1.8±0.24 μM, and 2.9±0.12 μM, respectively).VBIT-4 offer a therapeutic strategy for treating different diseases associated with enhanced apoptosis and point to VDAC1 as a promising target for therapeutic intervention.
In vivo	METHODS: To test the effect on Alzheimer's disease, VBIT-4 (25 mg/kg) was injected intraperitoneally into APP/PS1 mice once daily for four weeks. RESULTS: VDAC1 was significantly up-regulated in APP/PS1 mice and decreased after VBIT-4 treatment; GPX4 expression was decreased in APP/PS1 mice and restored by VBIT-4 treatment; MWM test showed that APP/PS1 mice had a longer latency to find the plateau; VBIT-4 treatment significantly decreased the latency to find the plateau in APP/PS1 mice. VBIT-4 treatment significantly reduced the latency of platform finding in APP/PS1 mice. [3]

Chemical Properties

Molecular Weight	457.87
Formula	C₂₁H₂₃ClF₃N₃O₃
Cas No.	2086257-77-2
Smiles	C(CC(NC1=CC=C(Cl)C=C1)=O)(CO)N2CCN(CC2)C3=CC=C(OC(F)(F)F)C=C3
Relative Density.	no data available

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 245 mg/mL (535.09 mM), Sonication is recommended.

In Vivo Formulation

5% DMSO+40% PEG300+5% Tween 80+50% Saline: 2 mg/mL (4.37 mM)

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4.5 mg/mL (9.83 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1840 mL	10.9201 mL	21.8403 mL	109.2013 mL
5 mM	0.4368 mL	2.1840 mL	4.3681 mL	21.8403 mL
10 mM	0.2184 mL	1.0920 mL	2.1840 mL	10.9201 mL
20 mM	0.1092 mL	0.5460 mL	1.0920 mL	5.4601 mL
50 mM	0.0437 mL	0.2184 mL	0.4368 mL	2.1840 mL
100 mM	0.0218 mL	0.1092 mL	0.2184 mL	1.0920 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc