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Tetrahydrouridine dihydrate

Catalog No. T60553

Tetrahydrouridine dihydrate (THU dihydrate) is a potent cytidine deaminase (CDA) inhibitor. Tetrahydrouridine dihydrate competitively blocks the active site of the enzyme more effectively than intrinsic cytidine.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Tetrahydrouridine dihydrate Chemical Structure

Tetrahydrouridine dihydrate, CAS N/A

Pack Size	Availability	Price/USD
25 mg	10-14 weeks	$ 1,520.00
50 mg	10-14 weeks	$ 1,980.00
100 mg	10-14 weeks	$ 2,500.00

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This compound is a customized synthesis product. We have a strong synthesis team with excellent synthesis technology and capabilities. However, due to various objective factors, there is a low probability that the synthesis will not be successful. If you need to learn more, please feel free to consult us, we will serve you wholeheartedly.

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Tetrahydrouridine dihydrate (THU dihydrate) is a potent cytidine deaminase (CDA) inhibitor. Tetrahydrouridine dihydrate competitively blocks the active site of the enzyme more effectively than intrinsic cytidine.
In vitro	Tetrahydrouridine (THU), a specific cytidine deaminase (CDA) inhibitor, effectively prevents the deamination process in the metabolism of cytotoxic deoxycytidine analogues, such as ara-C and Gemcitabine. Research involving combination therapy demonstrates that THU enhances Gemcitabine's anti-cancer efficacy against pancreatic and lung carcinoma cells. Notably, BxPC-3 and H441 cells, with high CDA expression, exhibit significantly increased sensitivity to Gemcitabine following a 100 μM THU treatment, with sensitivity augmenting by approximately 2.1 and 4.4 fold, respectively. Conversely, MIAPaCa-2 and H1299 cells, characterized by low CDA expression, also show unexpected sensitivity increases to Gemcitabine, with a 2.2 and 2.3 fold enhancement in IC 50 respectively. However, Panc-1 and H322 cells display no notable change in drug responsiveness. These findings indicate that THU is capable of sensitizing certain pancreatic and lung carcinoma cells to Gemcitabine, leading to cell death, independently of CDA expression levels, and does so by halting the S-phase of the cell cycle without inducing apoptosis [1].
In vivo	Administration of 167 mg/kg Tetrahydrouridine (THU) followed by 1.0 mg/kg DAC led to the death of one male and eight females. Surviving animals, scheduled for termination, were mostly asymptomatic, exhibiting no treatment-related effects on body weights, food consumption, clinical chemistry, and urinalysis when treated with up to 1.0 mg/kg DAC in combination with 167 mg/kg Tetrahydrouridine [2].

Molecular Weight	284.26
Formula	C9H20N2O8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

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You can also refer to dose conversion for different animals. More

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Tetrahydrouridine dihydrate inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.