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SR18662

Name: SR18662
Brand: TargetMol
SKU: T22429
Price: 41 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T22429

SR18662, an optimized compund based on ML264 that inhibits Krüppel-like factor 5 (KLF5) with IC50 of 4.4 nM, reduces the viability of multiple colorectal cancer cell lines and induces apoptosis.

SR18662

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Purity: 99.2%

Catalog No. T22429

SR18662, an optimized compund based on ML264 that inhibits Krüppel-like factor 5 (KLF5) with IC50 of 4.4 nM, reduces the viability of multiple colorectal cancer cell lines and induces apoptosis.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$41	In Stock	In Stock
5 mg	$97	In Stock	In Stock
10 mg	$163	In Stock	In Stock
25 mg	$328	In Stock	In Stock
50 mg	$528	In Stock	In Stock
100 mg	$756	In Stock	In Stock
200 mg	$987	In Stock	In Stock
500 mg	$1,530	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$107	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.2%

Appearance:Solid

Color:White

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	SR18662, an optimized compund based on ML264 that inhibits Krüppel-like factor 5 (KLF5) with IC50 of 4.4 nM, reduces the viability of multiple colorectal cancer cell lines and induces apoptosis.
Targets&IC50	KLF5:4.4 nM
In vitro	SR18662 significantly reduces growth and proliferation of CRC cells and shows improved efficacy in reducing viability of multiple CRC cell lines. Flow cytometry analysis following SR18662 treatment shows an increase in cells captured in either S or G2/M phases of the cell cycle and a significant increase in the number of apoptotic cells. SR18662 also reduces the expression of cyclins and components of WNT and MAPK signaling pathways.[1]
In vivo	The effect of SR18662 treatment shows a significant dose-dependent inhibition of xenograft growth in mice and exceeds ML264 at equivalent doses. SR18662 is potential for colorectal cancer therapy.[1]
Cell Research	Cell lines: HT29, HCT116, DLD-1 and SW620 colorectal cancer cell lines. Concentrations: 0.001 μM-20 μM. Incubation Time: 24 h. Method: KLF5 promoter activity assay: DLD-1/pGL4.18hKLF5p cells are seeded in 96 well plate and treated with SR18662 dissolved in DMSO in the range of 0.001 to 20 μM or equivalent volume of DMSO as control for 24 h, the human KLF5 promoter activity is determined with the ONE-Glo luciferase assay system by a SpectramMax M3 plate reader.
Animal Research	Animal Models: 7-week-old male NuJ/Foxn1nu mice with DLD-1 human colorectal cells injected. Dosages: 5 mg/kg, 10 mg/kg, 25 mg/kg. Administration: I.P.

Chemical Properties

Molecular Weight	420.31
Formula	C₁₆H₁₉Cl₂N₃O₄S
Smiles	ClC1=C(Cl)C=C(/C=C/C(NCC(N2CCN(S(=O)(C)=O)CC2)=O)=O)C=C1
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 45 mg/mL (107.06 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3792 mL	11.8960 mL	23.7920 mL	118.9598 mL
5 mM	0.4758 mL	2.3792 mL	4.7584 mL	23.7920 mL
10 mM	0.2379 mL	1.1896 mL	2.3792 mL	11.8960 mL
20 mM	0.1190 mL	0.5948 mL	1.1896 mL	5.9480 mL
50 mM	0.0476 mL	0.2379 mL	0.4758 mL	2.3792 mL
100 mM	0.0238 mL	0.1190 mL	0.2379 mL	1.1896 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc