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Pirodavir

Catalog No. T1750 CAS 124436-59-5

Synonyms: R77975

Pirodavir (R77975) (R 77975), the prototype of broad-spectrum anti-picornavirus compounds, is a potent human rhinovirus (HRV) capsid-binding inhibitor.

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Pirodavir, CAS 124436-59-5

Pack Size	Availability	Price/USD
1 mg	In stock	$ 91.00
2 mg	In stock	$ 147.00
5 mg	In stock	$ 225.00
10 mg	In stock	$ 312.00
25 mg	In stock	$ 541.00
50 mg	In stock	$ 778.00
100 mg	In stock	$ 1,090.00
500 mg	In stock	$ 2,180.00
1 mL * 10 mM (in DMSO)	In stock	$ 225.00

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Purity: 99.78%

Purity: 98.66%

Purity: 98.43%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Pirodavir (R77975) (R 77975), the prototype of broad-spectrum anti-picornavirus compounds, is a potent human rhinovirus (HRV) capsid-binding inhibitor.
In vitro	Pirodavir is a potent, broad-spectrum picornavirus inhibitor demonstrating significant efficacy against a wide range of human rhinoviruses (HRV) and enteroviruses. It effectively inhibits 80 of 100 tested HRV strains at a minimal concentration of 64 ng/mL and shows comparable activity against 16 enteroviruses with an 80% inhibitory concentration (IC80) averaged at 1,300 ng/mL. Notably, Pirodavir also suppresses enterovirus 71 replication, evidenced by an IC50 of 5,420 nM and an IC90 exceeding 13,350 nM. Its broad antiviral spectrum extends to 56 rhinovirus laboratory strains and three clinical isolates, achieving inhibition of 59% of the tested serotypes and isolates at IC50 values below 100 nM. In cytotoxicity studies, Pirodavir concentrations of 16 and 4μg/mL resulted in a reduction of cell growth by 66% and 28%, respectively, with higher tolerance observed in confluent HeLa cells at 33°C, where the 50% cytotoxic concentration surpasses 50μg/mL, compared to 7μg/mL for logarithmic cell growth at 37°C. These findings underscore Pirodavir's efficacy as a selective inhibitor for a significant fraction of picornaviruses with minimal cytotoxic effects under specified assay conditions.
Kinase Assay	The extract and binding assay buffer consists of 25 mM sodium phosphate, 10 mM potassium fluoride, 10 mM sodium molybdate, 10% glycerol, 1.5 mM EDTA, 2 mM dithiothreitol, 2 mM CHAPS, and 1 mM phenylmethylsulfonyl fluoride (pH 7.4), at room temperature. Intracellular receptors produced in this fashion exhibit reproducible interaction with known ligands at the published affinity. These preparations are subjected to extensive quality control experiments before the assays, covering receptor response, specificity, size, and reference ligand affinity. Receptor assays are performed with a final volume of 250 μL containing from 50-75 μg of extract protein, plus 1-2 nM [3H]Dex at 84 Ci/mmol and varying concentrations of competing ligand (0 to 10 μM). Assays are set up using a 96-well minitube system, and incubations are carried out at 4°C for 18 h. Equilibrium under these conditions of buffer and temperature is achieved by 6-8 h. Nonspecific binding is defined as that binding remaining in the presence of 1000 nM unlabeled Dex. At the end of the incubation period, 200 μL of 6.25% hydroxyapatite are added in wash buffer (binding buffer in the absence of dithiothreitol and phenylmethylsulfonyl fluoride). Specific ligand binding to receptor is determined by a hydroxyapatite-binding assay. Hydroxyapatite absorbs the receptor-ligand complex, allowing for the separation of bound from free radiolabeled ligand. The mixture is vortexed and incubated for 10 min at 4°C and centrifuged, and the supernatant is removed. The hydroxyapatite pellet is washed two times in wash buffer. The amount of receptor-ligand complex is determined by liquid scintillation counting of the hydroxyapatite pellet after the addition of 0.5 mM EcoScint A scintillation cocktail from National Diagnostics[1].
Cell Research	Pirodavir (R 77975) is dissolved in DMSO (10 mg/mL) and stored, and then diluted in growth medium before use[2]. HeLa cells are seeded at a concentration of approximately 180,000 cells per dish in six-well plates containing 4 mL of growth medium. Growth medium consist of Eagle's basal medium, supplemented with 5% fetal calf serum, 2% sodium bicarbonate, and 1% glutamine. After 24 h of incubation at 37°C in a humidified CO2 atmosphere, the growth medium is removed and replaced by the test solutions (fresh growth medium with or without various concentrations of the antiviral compounds). To assess the cytotoxicity of the antiviral compounds (e.g., Pirodavir), the number of living cells are determined present in triplicate cultures at the time of Pirodavir addition and every 24 h for 3 days. Following trypsinization, the number of viable cells for each drug concentration is counted in triplicate with a Coulter Counter[2].

Synonyms	R77975
Molecular Weight	369.46
Formula	C21H27N3O3
CAS No.	124436-59-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 11 mg/mL (29.77 mM)

References and Literature

1. Barnard DL, et al. In vitro activity of expanded-spectrum pyridazinyl oxime ethers related to pirodavir: novel capsid-binding inhibitors with potent antipicornavirus activity. Antimicrob Agents Chemother. 2004 May;48(5):1766-72. 2. Andries K, et al. In vitro activity of pirodavir (R 77975), a substituted phenoxy-pyridazinamine with broad-spectrum antipicornaviralactivity. Antimicrob Agents Chemother. 1992 Jan;36(1):100-7.

Related compound libraries

This product is contained In the following compound libraries：

Drug Repurposing Compound Library Inhibitor Library Anti-Viral Compound Library Clinical Compound Library NO PAINS Compound Library Bioactive Compounds Library Max Nonsteroidal Anti-Inflammatory Compound Library Anti-Infection Compound Library ReFRAME Related Library Anti-COVID-19 Compound Library

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Keywords

Pirodavir 124436-59-5 Immunology/Inflammation Microbiology/Virology Antiviral Virus Protease Rhinovirus R77975 inhibit R 77975 Enterovirus HEVs HEV Inhibitor HRVs HRV R-77975 inhibitor

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