Home Tools
Log in
Cart

OSMI-1

Catalog No. T16409   CAS 1681056-61-0

OSMI-1 is an O-GlcNAc transferase (OGT) inhibitor (IC50=2.7 μM) that is orally active and cell-permeable. OSMI-1 inhibits protein O-GlcNA acetylation without qualitatively altering cell-surface N- or O- linked glycans.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
OSMI-1 Chemical Structure
OSMI-1, CAS 1681056-61-0
Pack Size Availability Price/USD Quantity
1 mg In stock $ 34.00
2 mg In stock $ 48.00
5 mg In stock $ 77.00
10 mg In stock $ 126.00
25 mg In stock $ 207.00
50 mg In stock $ 369.00
100 mg In stock $ 549.00
1 mL * 10 mM (in DMSO) In stock $ 88.00
Bulk Inquiry
Get quote
Select Batch  
Purity: 99.83%
ee: 97.18%
Purity: 99.04%
Purity: 96.79%
Contact us for more batch information
Biological Description
Chemical Properties
Storage & Solubility Information
Description OSMI-1 is an O-GlcNAc transferase (OGT) inhibitor (IC50=2.7 μM) that is orally active and cell-permeable. OSMI-1 inhibits protein O-GlcNA acetylation without qualitatively altering cell-surface N- or O- linked glycans.
Targets&IC50 OGT:2.7 μM
In vitro METHODS: COS7 and Hela cells were treated with OSMI-1 (2-100 µM) for 24 h. Cell viability was measured using the CCK8 Assay.
RESULTS: 50 µM OSMI-1 reduced cell viability by more than 50%. [1]
METHODS: CHO cells were treated with OSMI-1 (10-100 µM) for 24 h, and the expression levels of target proteins were detected by Western Blot.
RESULTS: OSMI-1 reduced global OGlcNAcylation in a dose-dependent manner, with a maximum effect at 50 µM. [2]
In vivo METHODS: To assay antitumor activity in vivo, OSMI-1 (1 mg/kg, i.v.) and TRAIL (500 µg/kg, intraperitoneally) were administered to BALB/c-Foxn1nu/ArcGem nude mice harboring human colorectal carcinoma tumor HCT116 once daily for three weeks.
RESULTS: Tumor size was slightly reduced in mice treated with OSMI-1 or TRAIL alone, but significantly reduced in the OSMI-1 and TRAIL combination group. The combination treatment synergistically increased the antitumor activity of transplanted tumors in HCT116 nude mice. [3]
METHODS: To investigate the protective effect against Stx-mediated pathogenic responses, OSMI-1 (300-1000 µg/mouse in water containing 4.5% DMSO and 5% Tween 80) was injected intraperitoneally into Stx2a-attacked C57BL/6 mice once daily for seven days.
RESULTS: O-GlcNAc inhibition ameliorated the mortality and various disease symptoms induced by Stx2a exposure in mice, which was further enhanced by O-GlcNAc inhibition. [4]
Molecular Weight 563.64
Formula C28H25N3O6S2
CAS No. 1681056-61-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 83.33 mg/mL (147.84 mM), sonification is recommended.

TargetMolReferences and Literature

1. Liu Y, et al. Discovery of a Low Toxicity O-GlcNAc Transferase (OGT) Inhibitor by Structure-based Virtual Screening of Natural Products. Sci Rep. 2017 Sep 26;7(1):12334. 2. Ortiz-Meoz RF, et al. A small molecule that inhibits OGT activity in cells. ACS Chem Biol. 2015 Jun 19;10(6):1392-7. 3. Lee SJ, et al. OSMI-1 Enhances TRAIL-Induced Apoptosis through ER Stress and NF-κB Signaling in Colon Cancer Cells. Int J Mol Sci. 2021 Oct 14;22(20):11073. 4. Lee KS, et al. Inhibition of O-GlcNAcylation protects from Shiga toxin-mediated cell injury and lethality in host. EMBO Mol Med. 2022 Jan 11;14(1):e14678.

TargetMolCitations

1. Wang N, Yu M, Fu Y, et al. Blocking ATM Attenuates SKOV3 Cell Proliferation and Migration by Disturbing OGT/OGA Expression via hsa-miR-542-5p. Frontiers in Oncology. 2022.12 2. Liu R, Liu Y, Zhang W, et al.PCK1 attenuates tumor stemness via activating the Hippo signaling pathway in hepatocellular carcinoma.Genes & Diseases.2023: 101114. 3. Wang J, Aniwan A, Liu H, et al.O-GlcNAcylation regulates HIF-1α and induces mesothelial-mesenchymal transition and fibrosis of human peritoneal mesothelial cells.Heliyon.2023

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Metabolism Compound Library Inhibitor Library Bioactive Compounds Library Max NO PAINS Compound Library

Related Products

Related compounds with same targets
RHC 80267 Cyclandelate Glabrol Rimonabant Lecimibide 2-Furoic acid RP-64477 BMS-986172

TargetMolDose Conversion

You can also refer to dose conversion for different animals. More

TargetMol In vivo Formulation Calculator (Clear solution)

Step One: Enter information below
Dosage
mg/kg
Average weight of animals
g
Dosing volume per animal
ul
Number of animals
Step Two: Enter the in vivo formulation
% DMSO
%
% Tween 80
% ddH2O
Calculate Reset

TargetMolCalculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculation
Molecular Weight Calculator
=
X
X

Molarity Calculator allows you to calculate the

  • Mass of a compound required to prepare a solution of known volume and concentration
  • Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Concentration of a solution resulting from a known mass of compound in a specific volume
See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

X
=
X

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

=
/

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

OSMI-1 1681056-61-0 Metabolism Others Acyltransferase cell-permeable Inhibitor OGT Diacylglycerol acyltransferase O-GlcNAcylation toxicity mammalian inhibit zebrafish OSMI1 OSMI 1 acyl-CoA:cholesterol acyltransferase Diglyceride acyltransferase O-GlcNAc mono- acylglycerol acyltransferase transferase inhibitor

 

TargetMol