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M344

Catalog No. T2157 CAS 251456-60-7

Synonyms: MS 344, Histone Deacetylase Inhibitor III

M344 (Histone Deacetylase Inhibitor III) is a potent HDAC inhibitor with IC50 of 100 nM and able to induce cell differentiation.

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M344, CAS 251456-60-7

Pack Size	Availability	Price/USD
5 mg	In stock	$ 47.00
10 mg	In stock	$ 82.00
25 mg	In stock	$ 175.00
50 mg	In stock	$ 325.00
100 mg	In stock	$ 485.00
1 mL * 10 mM (in DMSO)	In stock	$ 52.00

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Purity: 99.95%

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Description	M344 (Histone Deacetylase Inhibitor III) is a potent HDAC inhibitor with IC50 of 100 nM and able to induce cell differentiation.
Targets&IC50	HDAC:100 nM
Kinase Assay	Enzyme Inhibition : Radioactively labeled chicken core histones are used as the enzyme substrate. The enzyme liberated tritiated acetic acid from the substrate which is quantitated by scintillation counting. IC50 values are results of triple determinations. 50 μL of maize enzyme (at 30 °C) is incubated (30 minutes) with 10 μL of total [3H]acetate-prelabeled chicken reticulocyte histones (1 mg/mL). Reaction is stopped by addition of 36 μL of 1 M HCl/0.4 M acetate and 800 μL of ethyl acetate. After centrifugation (10000 g, 5 minutes) an aliquot of 600 μL of the upper phase is counted for radioactivity in 3 mL of liquid scintillation cocktail. M344 is tested in a starting concentration of 40 μM, and active substances are diluted further.
Cell Research	MEL DS19 cells (murine erythroleukemia cells) are maintained in D-MEM containing 100 units/mL penicillin G sodium and 100 μg/mL streptomycin sulfate supplemented with 10% fetal bovine serum at 37 °C in a 5% CO2 atmosphere. To test M344 for potential to induce cell differentiation, log-phase cells with a population doubling time of 11−13 hours are used. Serial dilutions of M344 are prepared in 24-well plates using 1 mL of D-MEM/well. If M344 are dissolved in DMSO, control wells contains the same amount of solvent (generally 2 μL/mL of medium). Subsequently, the cell suspension is added to the wells. After 72 hours the experiment is evaluated. Cell numbers are counted using a Casy 1 TTC flow cytometer. The proliferation of treated cells is expressed as percent proliferation in comparison with the solvent control. Differentiated MEL cells accumulate hemoglobin. Therefore, the induction of cell differentiation is determined by benzidine staining according to the literature. To 100 μL of cell suspension is added 10 μL of a 0.4% solution of benzidine in 12% acetic acid containing 2% Water2. Within 5 minutes hemoglobin-containing cells stains blue. Benzidine-positive and -negative cells are counted under the microscope in a hemocytometer, and the percentage of positive cells is calculated. M344 is first tested at 10 μM and 50 μM final concentration. According to activity/toxicity profile, a range of concentrations is chosen for a dose−response analysis. (Only for Reference)

Synonyms	MS 344, Histone Deacetylase Inhibitor III
Molecular Weight	307.39
Formula	C16H25N3O3
CAS No.	251456-60-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 4 mg/mL (13.01 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 62 mg/mL (201.69 mM)

References and Literature

1. Jung M, et al. J Med Chem. 1999, 42(22), 4669-4679. 2. Takai N, et al. Gynecol Oncol. 2006, 101(1), 108-113. 3. Furchert SE, et al. Int J Cancer. 2007, 120(8), 1787-1794. 4. St Germain C, et al. Cancer Cell Int. 2010,10, 32.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Aging Compound Library Autophagy Compound Library Cancer Cell Differentiation Compound Library Anti-Cancer Compound Library Apoptosis Compound Library Reprogramming Compound Library Anti-COVID-19 Compound Library Chromatin Modification Compound Library HIF-1 Signaling Pathway Compound Library

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Related compounds with same targets

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Keywords

M344 251456-60-7 Chromatin/Epigenetic DNA Damage/DNA Repair HDAC inhibit Inhibitor MS-344 D-237 MS 344 Histone Deacetylase Inhibitor III D237 M-344 D 237 M 344 Histone deacetylases MS344 inhibitor

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