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JSH-23

Catalog No. T1930 CAS 749886-87-1

JSH-23, an NF-κB inhibitor（IC50= 7.1 μM）, suppresses the transcriptional activity.

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JSH-23, CAS 749886-87-1

Pack Size	Availability	Price/USD
1 mg	In stock	$ 36.00
2 mg	In stock	$ 52.00
5 mg	In stock	$ 85.00
10 mg	In stock	$ 122.00
25 mg	In stock	$ 197.00
50 mg	In stock	$ 297.00
100 mg	In stock	$ 453.00
1 mL * 10 mM (in DMSO)	In stock	$ 94.00

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Purity: 99.67%

Purity: 98.03%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	JSH-23, an NF-κB inhibitor（IC50= 7.1 μM）, suppresses the transcriptional activity.
Targets&IC50	NF-κB:7.1 μM
In vitro	Within diabetic rat models, JSH-23 (3 mg/kg) effectively reduces neuroinflammation and enhances antioxidant defenses, significantly reversing neural conduction impairments and ultimately ameliorating insufficient neurovascular perfusion.
In vivo	In RAW 264.7 cells, JSH-23 at concentrations below 100 μM did not exhibit significant cytotoxic effects and was capable of inhibiting lipopolysaccharide-(LPS)-induced apoptotic chromatin condensation. Furthermore, in primary cerebellar neurons of mice, JSH-23 inhibited the LPS-induced nuclear translocation of NF-κB p65 without affecting the degradation of IκBα.
Kinase Assay	Measurement of NF-κB transcriptional activity: Macrophages RAW 264.7 transfected stably with reporter plasmid of pNF-κB-SEAP-NPT are treated with 1 μg/ml LPS and/or sample for 16 hours. As the reporter, SEAP activity in the cell-free culture media is measured as followed. Single cell-derived stable transfectants are plated in 5 ml of T-25 flask, and the media is decanted 24 h later. At this time, cells are washed twice with phosphate-buffered saline, and incubations are initiated by addition of new media. Chemicals are added to the culture medium after 24 h of incubations. Aliquots (25 ml) of medium from a control or chemical-treated cultures are taken at 0, 3, 20, 24, 48, and 72 h, heated at 65°C for 5 min to eliminate the alkaline phosphatase activity, and used immediately or stored at -20°C. Mixtures consisting of dilution buffer (25 ml), assay buffer (97 ml), culture media (25 ml), and 4-methylumbelliferyl phosphate (MUP, 1 mM, 3 ml) in each well of the 96-well plates are incubated for 60 min in the dark at room temperature. Fluorescence emits the product of the SEAP/MUP is measured at 449 nm using a 96-well plate fluorometer after excitation at 360 nm.
Cell Research	Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 compound for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.(Only for Reference)

Molecular Weight	240.34
Formula	C16H20N2
CAS No.	749886-87-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 45 mg/mL (187.2 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 16 mg/mL (66.6 mM)

References and Literature

1. Shin HM, et al. FEBS Lett. 2004, 571(1-3), 50-54. 2. Arias-Salvatierra D, et al. Cell Signal. 2011, 23(2), 425-435. 3. Kasparkova J, et al. FEBS J. 2014, doi: 10.1111/febs.12711. 4. Kumar A, et al. Diabetes Obes Metab. 2011, 13(8), 750-758. 5. Song H, Tang X, Li X, et al. HLJ2 Effectively Ameliorates Colitis-Associated Cancer via Inhibition of NF-κB and Epithelial–Mesenchymal Transition[J]. Drug Design, Development and Therapy. 2020, 14: 4291. 6. Lai X, Wang M, Zhu Y, et al. ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting inflammation and keratinocyte apoptosis via nuclear translocation of phosphorylated NF-κB p65 and cysteine deficiency[J]. Journal of Hazardous Materials. 2020: 124566.

Citations

1. Chen J, Liu G, Wang X, et al. Glioblastoma stem cell-specific histamine secretion drives pro-angiogenic tumor microenvironment remodeling. Cell Stem Cell. 2022 2. Lai X, Wang M, Zhu Y, et al. ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting inflammation and keratinocyte apoptosis via nuclear translocation of phosphorylated NF-κB p65 and cysteine deficiency. Journal of Hazardous Materials. 2020: 124566 3. Zhou D, Yang S, Yan H, et al. SC75741, a novel c-Abl inhibitor, promotes the clearance of TDP25 aggregates via ATG5-dependent autophagy pathway. Frontiers in Pharmacology. 2021: 2891. 4. Xia H, Zhang Z, You F. Inhibiting ACSL1-Related Ferroptosis Restrains Murine Coronavirus Infection. Viruses. 2021, 13(12): 2383. 5. Song H, Tang X, Li X, et al. HLJ2 Effectively Ameliorates Colitis-Associated Cancer via Inhibition of NF-κB and Epithelial–Mesenchymal Transition. Drug Design, Development and Therapy. 2020, 14: 4291 6. Zhang Q, Qian Y, Ren Y, et al.Phenethyl isothiocyanate inhibits metastasis potential of non-small cell lung cancer cells through FTO mediated TLE1 m6A modification.Acta Pharmacologica Sinica.2023: 1-14.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Prostate Cancer Compound Library NF-κB Signaling Compound Library Anti-Liver Cancer Compound Library Bioactive Compound Library Oxidation-Reduction Compound Library Transcription Factor-Targeted Compound Library Immunology/Inflammation Compound Library Bioactive Compounds Library Max Anti-Aging Compound Library

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Keywords

JSH-23 749886-87-1 NF-Κb NF-κB Nuclear factor-κB Nuclear factor-kappaB Inhibitor JSH 23 JSH23 inhibit inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.