Powder: -20°C for 3 years | In solvent: -80°C for 1 year
IRAK-1-4 Inhibitor I is a dual inhibitor of IRAK4 and IRAK1.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 37.00 | |
2 mg | In stock | $ 50.00 | |
5 mg | In stock | $ 72.00 | |
10 mg | In stock | $ 112.00 | |
25 mg | In stock | $ 258.00 | |
50 mg | In stock | $ 486.00 | |
100 mg | In stock | $ 695.00 | |
500 mg | In stock | $ 1,490.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 72.00 |
Description | IRAK-1-4 Inhibitor I is a dual inhibitor of IRAK4 and IRAK1. |
Targets&IC50 | IRAK1:0.2 μM, IRAK4:0.3 μM |
In vitro | IRAK-1-4 Inhibitor I has IC50 greater than the highest concentration tested (10 μM) against a panel of 27 other kinases, including the most closely homologous (outside of the IRAK family) Lck and pp60SRC. Additionally, IRAK-1-4 Inhibitor I does not show any signs of cytotoxicity in a 72 h proliferation assay in HeLa cells (ED50>30 μM). Significant inhibition of IRAK-1 is observed with IRAK-1-4 Inhibitor I (IRAK-1 IC50=0.3 μM)[1]. IRAK-1/4 inhibitor eliminates the LPS-induced increases in Bcl10, NF-κB, and IL-8. IRAK-1/4 mediates LPS-induced IL-8 activation and functions upstream of Bcl10. The LPS-induced increase in Bcl10 declines by 73% (from 5.18±0.22 to 2.36±0.08 ng/mL), and the IL-8 response decline by 60% (from 2.64±0.31 to 1.14±0.08 ng/mL)[2]. |
Kinase Assay | Kinase Assays: Cell-free kinase assays are done in quadruplicate with 1 μM ATP to determine the IC50 values of AV-951 against a variety of recombinant receptor and nonreceptor tyrosine kinases including VEGFR1, VEGFR2, VEGFR3, c-Kit, PDGFRβ, Flt-3 and FGFR1. |
Cell Research | IRAK-1-4 Inhibitor I is dissolved in DMSO and stored, and then diluted with appropriate media before use[2]. NCM460 cells, grown in 24-well plates, are incubated with 50 μM IRAK-1/4 inhibitor for 2 h. After 2 h, the media are changed, and new media with or without LPS (10 ng/mL) added. Treatment is terminated at 6 h, and spent media and cells are collected for IL-8 and other assays[2]. |
Synonyms | IRAK-1/4 Inhibitor I, IRAK-1/4 Inhibitor |
Molecular Weight | 395.41 |
Formula | C20H21N5O4 |
CAS No. | 509093-47-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 7.9 mg/mL(20 mM)
You can also refer to dose conversion for different animals. More
bottom
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
IRAK-1-4 Inhibitor I 509093-47-4 Immunology/Inflammation NF-Κb IRAK IRAK-1/4 Inhibitor I inhibit Interleukin-1 receptor associated kinase IRAK 1 4 Inhibitor I IRAK-1/4 Inhibitor IRAK14 Inhibitor I IL-1R associated kinase Inhibitor inhibitor