Powder: -20°C for 3 years | In solvent: -80°C for 1 year
HC-030031 (TOSLAB 829227) is a potent TRPA1 inhibitor, which antagonizes AITC- and formalin-evoked calcium influx with IC50s of 6.2±0.2 and 5.3±0.2 μM, respectively.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
10 mg | In stock | $ 46.00 | |
50 mg | In stock | $ 97.00 | |
100 mg | In stock | $ 148.00 | |
200 mg | In stock | $ 195.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 48.00 |
Description | HC-030031 (TOSLAB 829227) is a potent TRPA1 inhibitor, which antagonizes AITC- and formalin-evoked calcium influx with IC50s of 6.2±0.2 and 5.3±0.2 μM, respectively. |
Targets&IC50 | AITC--evoked calcium influx:6.2±0.2 μM, formalin-evoked calciμM influx:5.3±0.2 μM |
Kinase Assay | The activity of specific TLR agonists is assessed using the secretory embryonic alkaline phosphatase (SEAP) reporter gene that is linked to NF-κB activation in response to TLR stimulation. Measurement of SEAP activity using the Quanti-blue substrate (InvivoGen) after TLR agonist treatment is carried out. |
Cell Research | HC-030031 is prepared in DMSO and stored, and then diluted with appropriate medium (DMSO 0.4%) before use[2]. HEK-293 cells stably expressing human TRPA1 are plated into 384-well plates at a density of 20,000 cells/well 24 hours prior to assaying. On the day of assay, cells are loaded with 4 μM Fluo-4 dye and 0.08% pluronic acid for 1 hour at room temperature in assay buffer consisting of Hank's balanced salt solution supplemented with 20 mM HEPES, 2.5 mM probenecid, and 4% TR-40. Calcium influx assays are performed using the Fluorometric Imaging Plate Reader (FLIPR) TETRA. Concentration-response curves are generated for the TRPA1 agonists cinnamaldehyde and AITC prior to antagonist testing so EC60 concentrations could be determined. Titrations of HC-030031 are made from a DMSO stock solution and DMSO is kept to a constant of 0.4% in the assay. The antagonist is incubated with the cells for 10 minutes before the addition of an EC60 concentration of either cinnamaldehyde (18 μM) or AITC (6 μM) and calcium influx is monitored for an additional 10 minutes[2]. |
Animal Research | Animal Models: Male Sprague-Dawley rats. Formulation: 0.5% Methylcellulose. Dosages: 100,300 mg/kg. Administration: oral administration |
Synonyms | HC030031, TOSLAB 829227 |
Molecular Weight | 355.39 |
Formula | C18H21N5O3 |
CAS No. | 349085-38-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 35.5 mg/mL (100 mM)
You can also refer to dose conversion for different animals. More
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HC-030031 349085-38-7 Membrane transporter/Ion channel TRP/TRPV Channel TRP Channel HC030031 Transient receptor potential channels TOSLAB 829227 Inhibitor HC 030031 TOSLAB829227 inhibit TOSLAB-829227 inhibitor