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Chrysophanol

Catalog No. T2995 CAS 481-74-3

Synonyms: 3-Methylchrysazin, Chrysophanic Acid, Turkey Rhubarb

Chrysophanol (Turkey Rhubarb) is an EGFR/mTOR pathway inhibitor, which can be found in rhubarb, and sorrel.

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Chrysophanol, CAS 481-74-3

Pack Size	Availability	Price/USD
50 mg	In stock	$ 32.00
100 mg	In stock	$ 48.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 99.7%

Purity: 99.44%

Purity: 97.91%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Chrysophanol (Turkey Rhubarb) is an EGFR/mTOR pathway inhibitor, which can be found in rhubarb, and sorrel.
In vivo	Chrysophanol (CA) has been shown to counteract obesity in C57BL/6 mice induced by a high-fat diet (HFD). Through in vivo studies involving male C57BL/6J mice, it was discovered that Chrysophanol significantly mitigates weight gain associated with HFD consumption. Specifically, mice subsisting on an HFD without Chrysophanol treatment experienced a weight increase of 23.92 ± 1.74 g over 16 weeks. In contrast, mice treated with Chrysophanol exhibited a markedly lower weight gain of 16.72 ± 2 g in the same period, underscoring Chrysophanol's potential as an effective intervention against HFD-induced obesity.
Cell Research	Chrysophanol (Chrysophanic Acid) is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. The cells are seeded at 5×103 cells/mL in 96-well microplates and allowed to attach for 24 h. Chrysophanol (20, 50, 80 and 120 μM) is added to the medium at various concentrations up to 120 μM and for different durations. After treatment, cell cytotoxicity and/or proliferation is assessed by a Cell Counting Kit-8 (CCK-8). Briefly, highly water-soluble tetrazolium salt, WST-8, produces an orange colored water-soluble product, formazan. The amount of formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells.CCK-8 (10 μL) is added to each well and incubated for 3 h at 37°C, then cell proliferation and cytotoxicity are assessed by measuring the absorbance at 450 nm using a microplate reader.Three replicated wells are used for each experimental condition[1].
Source	Natural Products > Polygonaceae > Rheum

Synonyms	3-Methylchrysazin, Chrysophanic Acid, Turkey Rhubarb
Molecular Weight	254.24
Formula	C15H10O4
CAS No.	481-74-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 5 mg/mL (19.66 mM), Heating is recommended.

References and Literature

1. Lee MS, et al. Phytother Res, 2011, 25(6), 833-837. 2. Semple SJ, et al. Antiviral Res, 2001, 49(3), 169-178. 3. Lim H, et al. Chrysophanic Acid Suppresses Adipogenesis and Induces Thermogenesis by Activating AMP-Activated Protein Kinase Alpha In vivo and In vitro. Front Pharmacol. 2016 Dec 8;7:476.

Citations

1. Zhang H, Cai J, Li C, et al.Wogonin inhibits latent HIV-1 reactivation by downregulating histone crotonylation.Phytomedicine.2023: 154855.

Related compound libraries

This product is contained In the following compound libraries：

Membrane Protein-targeted Compound Library Miao medicine Compound Library Drug Repurposing Compound Library Traditional Chinese Medicine Monomer Library Inhibitor Library FDA-Approved Kinase Inhibitor Library Kinase Inhibitor Library Tyrosine Kinase Inhibitor Library Anti-Cancer Approved Drug Library Anti-Cancer Drug Library

Related Products

Related compounds with same targets

PP1 AG490 EGFR-IN-99 Pertuzumab WAY-270360 SU5204 EGFR/ErbB-2/ErbB-4 inhibitor-2 WS6

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Keywords

Chrysophanol 481-74-3 Angiogenesis JAK/STAT signaling Tyrosine Kinase/Adaptors EGFR 3-Methylchrysazin Epidermal growth factor receptor ErbB-1 Chrysophanic Acid HER1 inhibit Turkey Rhubarb Inhibitor inhibitor

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