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Chrysophanol

Catalog No. T2995 CAS 481-74-3

Synonyms: Turkey Rhubarb, 大黄酚, 3-Methylchrysazin, Chrysophanic Acid

Chrysophanic acid is an EGFR/mTOR pathway inhibitor, which can be found in rhubarb, and sorrel.

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Chrysophanol, CAS 481-74-3

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Chrysophanic acid is an EGFR/mTOR pathway inhibitor, which can be found in rhubarb, and sorrel.
In vivo	Chrysophanol (CA) improves HFD-induced obesity in C57BL/6 Mice. The in vivo performance of Chrysophanol is performed in male C57BL/6J mice to determine the efficacy of administered Chrysophanol. Mice fed the HFD gained significantly more weight than those fed the standard diet mice. Furthermore, weight gain of Chrysophanol group is significantly less than with the untreated HFD. Mice in the HFD-group gained 23.92 ± 1.74 g of weight, while those in the Chrysophanol group gained 16.72±2 g of weight after 16 weeks[3].
Cell Research	Chrysophanol (Chrysophanic Acid) is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. The cells are seeded at 5×103 cells/mL in 96-well microplates and allowed to attach for 24 h. Chrysophanol (20, 50, 80 and 120 μM) is added to the medium at various concentrations up to 120 μM and for different durations. After treatment, cell cytotoxicity and/or proliferation is assessed by a Cell Counting Kit-8 (CCK-8). Briefly, highly water-soluble tetrazolium salt, WST-8, produces an orange colored water-soluble product, formazan. The amount of formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells.CCK-8 (10 μL) is added to each well and incubated for 3 h at 37°C, then cell proliferation and cytotoxicity are assessed by measuring the absorbance at 450 nm using a microplate reader.Three replicated wells are used for each experimental condition[1].

Synonyms	Turkey Rhubarb, 大黄酚, 3-Methylchrysazin, Chrysophanic Acid
Molecular Weight	254.241
Formula	C15H10O4
CAS No.	481-74-3

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

H2O: <1 mg/mL

Ethanol: <1 mg/mL

DMSO: 5 mg/mL (19.66 mM), warmed

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Lee MS, et al. Phytother Res, 2011, 25(6), 833-837. 2. Semple SJ, et al. Antiviral Res, 2001, 49(3), 169-178. 3. Lim H, et al. Chrysophanic Acid Suppresses Adipogenesis and Induces Thermogenesis by Activating AMP-Activated Protein Kinase Alpha In vivo and In vitro. Front Pharmacol. 2016 Dec 8;7:476.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Autophagy Compound Library Anti-Lung Cancer Compound Library HIF-1 Signaling Pathway Compound Library Anti-Cancer Metabolism Compound Library Cytokine Inhibitor Library Traditional Chinese Medicine Monomer Library Tibetan medicine Compound Library Anti-Obesity Compound Library Glutamine Metabolism Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Chrysophanol 481-74-3 JAK/STAT信号通路蛋白酪氨酸激酶血管生成 EGFR 3-Methylchrysazin Epidermal growth factor receptor ErbB-1 Turkey Rhubarb HER1 inhibit Inhibitor Chrysophanic Acid inhibitor

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