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Catalog No. T6209   CAS 1594094-64-0
Synonyms: CW069

CW069( IC50=75 μM), an allosteric selective inhibitor of microtubule motor protein HSET, exhibits remarkable specificity over <a href="/target/KSP" style="display: inline; color: #c13a36">KSP</a>.

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CW-069, CAS 1594094-64-0
Pack Size Availability Price/USD Quantity
2 mg In stock $ 72.00
5 mg In stock $ 101.00
10 mg In stock $ 166.00
25 mg In stock $ 295.00
50 mg In stock $ 369.00
100 mg In stock $ 693.00
1 mL * 10 mM (in DMSO) In stock $ 110.00
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Purity: 97.52%
Purity: 98.59%
Purity: 98.57%
Purity: 97.52%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description CW069( IC50=75 μM), an allosteric selective inhibitor of microtubule motor protein HSET, exhibits remarkable specificity over KSP.
Targets&IC50 HSET:75 μM
In vitro CW069 increases multipolar spindles in N1E-115 cells with supernumerary centrosomes without altering bipolar spindle morphology in normal human dermal fibroblast cells. CW069 inhibits growth in cancer N1E-115 cells with IC50 of 10 μM, but not in NHDF or primary human bone marrow cells. [1]
Kinase Assay In Vitro Enzymatic ATPase Assay: The protocol is optimized for use with full-length, N-terminal, 6His-tagged HSET and KSP, and measured the MT-stimulated activity of the proteins. Inhibition of the Gsp synthetase activity of HSET/KSP is observed spectrophotometrically by coupling the hydrolysis of ATP to oxidation of NADH via pyruvate kinase/lactate dehydrogenase reactions. The assay is initiated by adding purified Gsp synthetase/amidase (12.8 nM) to an assay mixture containing the following components (final concentration): 6 nM protein, 0.07 mg/ml MTs (University Biologicals), 1.56 mM glutathione, 10 mM spermidine, 2 mM ATP, 2.7 mM MgCl2, 1 mM phospho(enol)-pyruvate, 0.2 mM NADH, 50 μg/ml lactate dehydrogenase, 100 μg/ml pyruvate kinase, and various concentrations of inhibitor all in 50 mM Na PIPES (pH 6.8) at 37°C. The ADP-Glo detection assay (Promega) is performed as described in the manufacturer's instructions. All compound additions were performed using a multidrop BioMek Nxp. Plates were read using a Pherastar microplate reader.
Cell Research Cells are cultured in DMEM supplemented with 10% fetal calf serum (FCS) at 37°C and 5% CO2. All compounds used in the Sulforhodamine B colorimetric (SRB) assay are dissolved in DMSO and diluted in culture medium to a final concentration of 0.2% DMSO. For the SRB assay and live-cell imaging, cells are seeded in 96-well plates at a density of 2,500 cells per well. After 24 hr, the cells are treated with compound for 72 hr, with triplicate wells for each concentration. For the SRB assay, the cells are then fixed with trichloroacetic acid (TCA) and stained with SRB. Fluorescence is quantified using an Infinite 200 PRO plate-reader at a wavelength of 545 nm. Compound-treated wells are compared with solvent control wells and the concentration of compound that results in 50% of the solvent-control cell growth is designated as the IC50 concentration, calculated using Graphpad PRISM 6. At least three biological replicates are performed for each assay.(Only for Reference)
Synonyms CW069
Molecular Weight 500.336
Formula C23H21IN2O3
CAS No. 1594094-64-0


Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

Ethanol: 4 mg/mL (7.99 mM)

DMSO: 93 mg/mL (185.9 mM)

H2O: <1 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Watts CA, et al. Chem Biol. 2013, 20(11), 1399-1410.

Related compound libraries

This product is contained In the following compound libraries:
Microtubule-Targeted Compound Library Anti-Cancer Active Compound Library NO PAINS Compound Library Bioactive Compound Library Endoplasmic Reticulum Stress Compound Library Inhibitor Library Anti-Cancer Compound Library DNA Damage & Repair Compound Library Cytoskeletal Signaling Pathway Compound Library Bioactive Compounds Library Max

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CW-069 1594094-64-0 Cytoskeletal Signaling Kinesin Microtubule Associated CW069 Inhibitor inhibit CW 069 inhibitor