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BPR1J-097

Catalog No. T2272 CAS 1327167-19-0

Synonyms: BPR1J097

BPR1J-097) is a novel FLT-3 inhibitor(IC50: 11±7 nM) with promising in vivo anti-tumor activities. It also inhibits FLT-3 D835Y (IC50: 3 nM).

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BPR1J-097, CAS 1327167-19-0

Pack Size	Availability	Price/USD
2 mg	In stock	$ 50.00
5 mg	In stock	$ 63.00
10 mg	In stock	$ 108.00
25 mg	In stock	$ 237.00
50 mg	In stock	$ 405.00
100 mg	In stock	$ 585.00
1 mL * 10 mM (in DMSO)	In stock	$ 119.00

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Description	BPR1J-097) is a novel FLT-3 inhibitor(IC50: 11±7 nM) with promising in vivo anti-tumor activities. It also inhibits FLT-3 D835Y (IC50: 3 nM).
Targets&IC50	FLT3 (D835Y):3 nM, FLT3:11±7 nM
Kinase Assay	Kinase assays: To assay Plk1 and Aurora A kinase activity, cells are lysed after 13 hrs release in the presence of SBE13 after double thymidine block, and kinases are immunoprecipitated from lysates using antibodies as described. In brief, for each immunoprecipitation 800 μg of total protein were incubated with 1.5 μg Plk1 antibody cocktail, 3 μg Plk2 antibody, 3 μg Plk3 antibody, or 5 μg Aurora A antibody, respectively, for 2 hrs at 4°C on a rotator. Immunoprecipitated protein is collected using Protein G Agarose beads. The Plk1, Plk2 and Plk3 immunoprecipitates are incubated with 1 μg casein and with 1 μCi of [γ32-P]ATP for 30 min at 37°C in kinase buffer. The Aurora A immunoprecipitates are incubated with 0.5 μl Histone and with 1 μCi of [γ32-P]ATP for 60 min at room temperature in kinase buffer. Products from the kinase assays are fractionated on 10% Bis-Tris-polyacrylamide gels, and the phosphorylated substrate is visualized by autoradiography after an exposure of 12 to 36 hrs. An equal amount of immunoprecipitates is subjected to western blot analysis to confirm equal loading of Plk1, Plk2, Plk3 or Aurora A protein in kinase reactions. Immunoprecipitated Plk1 after 13 hrs release in the presence of SBE13 is assayed after de-phosphorylation using λ protein phosphatase and compared to kinase activity of endogenous immunoprecipitated Plk1. Activity of Plk1 kinase with and wiiiuithout de-phosphorylation is compared and the ratio between de-phosphorylated and "normal" endogenous immunoprecipitated Plk1 kinase activity is calculated.

Synonyms	BPR1J097
Molecular Weight	516.61
Formula	C27H28N6O3S
CAS No.	1327167-19-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 11 mg/mL (21.29 mM), Sonication is recommended.

References and Literature

1. Lin WH, et al. Br J Y. 2012 Jan 31;106(3):475-81.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Tyrosine Kinase Inhibitor Library Anti-Cancer Active Compound Library Bioactive Compound Library Kinase Inhibitor Library Anti-Cancer Compound Library Bioactive Compounds Library Max Anti-Lung Cancer Compound Library Angiogenesis related Compound Library Target-Focused Phenotypic Screening Library

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Keywords

BPR1J-097 1327167-19-0 Angiogenesis Tyrosine Kinase/Adaptors FLT CD135 Fms like tyrosine kinase 3 BPR-1J-097 FLT3 BPR1J097 Cluster of differentiation antigen 135 inhibit BPR1J 097 Inhibitor inhibitor

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