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ANNINE-6plus

Catalog No. T30078 CAS 1151942-85-6

ANNINE-6plus is a water soluble voltage sensitive dye (also called potentiometric dyes). This compound was developed at the Max Planck Institute for Biochemistry in Germany. It is used to optically measure the changes in transmembrane voltage of excitable cells, including neurons, skeletal and cardiac myocytes. ANNINE-6plus has a fractional fluorescent intensity change (ΔF/F per 100 mV change) of about 30% with single-photon excitation (~488 nm) and >50% with two-photon excitation (~1060 nm).

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ANNINE-6plus, CAS 1151942-85-6

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Biological Description

Chemical Properties

Storage & Solubility Information

Description

Molecular Weight	717.62
Formula	C39H47Br2N3
CAS No.	1151942-85-6

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

References and Literature

1. Ramamoorthy S, Wilson TM, Wu T, Nuttall AL. Non-uniform distribution of outer hair cell transmembrane potential induced by extracellular electric field. Biophys J. 2013 Dec 17;105(12):2666-75. doi: 10.1016/j.bpj.2013.11.021. PubMed PMID: 24359738; PubMed Central PMCID: PMC3882456. 2. Wegner LH, Frey W, Schönwälder S. A critical evaluation of whole cell patch clamp studies on electroporation using the voltage sensitive dye ANNINE-6. Bioelectrochemistry. 2013 Aug;92:42-6. doi: 10.1016/j.bioelechem.2013.03.002. Epub 2013 Apr 3. PubMed PMID: 23603150. 3. Pagès S, Côté D, De Koninck P. Optophysiological approach to resolve neuronal action potentials with high spatial and temporal resolution in cultured neurons. Front Cell Neurosci. 2011 Oct 10;5:20. doi: 10.3389/fncel.2011.00020. eCollection 2011. PubMed PMID: 22016723; PubMed Central PMCID: PMC3191737. 4. Vignali S, Peter N, Ceyhan G, Demir IE, Zeller F, Senseman D, Michel K, Schemann M. Recordings from human myenteric neurons using voltage-sensitive dyes. J Neurosci Methods. 2010 Oct 15;192(2):240-8. doi: 10.1016/j.jneumeth.2010.07.038. Epub 2010 Aug 5. PubMed PMID: 20691728.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

ANNINE-6plus 1151942-85-6 ANNINE 6plus ANNINE6plus inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.