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AM404

Catalog No. T39281 CAS 183718-77-6

AM404 is a chemical compound that functions as an inhibitor of endocannabinoid reuptake. It effectively blocks the transport of anandamide, with IC50 values measured in the low micromolar range. Moreover, AM404 exhibits the ability to induce relaxation in rat isolated hepatic arteries contracted with Phenylephrine. This relaxation effect is quantified by a pEC50 value of 7.4 (corresponding to an EC50 of 0.04 μM). Additionally, AM404 possesses a neuroprotective effect.

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AM404, CAS 183718-77-6

Pack Size	Availability	Price/USD
5 mg	35 days	$ 63.00
10 mg	35 days	$ 113.00
50 mg	35 days	$ 465.00
100 mg	35 days	$ 793.00

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Biological Description

Chemical Properties

Storage & Solubility Information

Description	AM404 is a chemical compound that functions as an inhibitor of endocannabinoid reuptake. It effectively blocks the transport of anandamide, with IC50 values measured in the low micromolar range. Moreover, AM404 exhibits the ability to induce relaxation in rat isolated hepatic arteries contracted with Phenylephrine. This relaxation effect is quantified by a pEC50 value of 7.4 (corresponding to an EC50 of 0.04 μM). Additionally, AM404 possesses a neuroprotective effect.
In vitro	AM404 reduces C6 glioma cell proliferation with IC 50 values of 4.9 μM. AM404 non-specifically inhibit C6 glioma cell proliferation at concentrations used to block the cellular accumulation of the endocannabinoid anandamide[2]. Cell Viability Assay[2]Cell Line: Rat C6 glioma cells Concentration: 1, 3, 10 and 30 μM Incubation Time: 24, 48, 72 and 96 h Result: Produced a concentration-dependent reduction in cell proliferation that was seen with 24 h of exposure to 10 and 30 μM concentrations and after 48 h at 3 μM. The lowest concentration of AM404 tested, 1 μM, produced a significant, albeit small, reduction in cell proliferation at 72 h.
In vivo	AM404 (1-5?mg/kg, i.p.) exerts dose-dependent anxiolytic-like effects in the three models: elevated plus maze, defensive withdrawal and separation-induced ultrasonic vocalizations. Animal Model: Adult male Sprague-Dawley rats (250-300?g)[2]Dosage: 2.5-10?mg/kg Administration: Intraperitoneal (i.p.) Result: Caused a dose-dependent increase in anandamide levels in prefrontal cortex, hippocampus and thalamus.

Molecular Weight	395.58
Formula	C26H37NO2
CAS No.	183718-77-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

References and Literature

1. A Giuffrida, et al. Mechanisms of endocannabinoid inactivation: biochemistry and pharmacology. J Pharmacol Exp Ther. 2001 Jul;298(1):7-14. 2. Kent-Olov Jonsson, et al. AM404 and VDM 11 non-specifically inhibit C6 glioma cell proliferation at concentrations used to block the cellular accumulation of the endocannabinoid anandamide. Arch Toxicol. 2003 Apr;77(4):201-7. 3. Marco Bortolato, et al. Anxiolytic-like properties of the anandamide transport inhibitor AM404. Neuropsychopharmacology. 2006 Dec;31(12):2652-9.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

AM404 183718-77-6 AM 404 AM-404 inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.