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6-Aminonicotinamide

Catalog No. T7545 CAS 329-89-5

6-Aminonicotinamide (6-AN) is a well-established inhibitor of the NADP+-dependent enzyme.

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6-Aminonicotinamide, CAS 329-89-5

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50 mg	In stock	$ 30.00
100 mg	In stock	$ 43.00
200 mg	In stock	$ 61.00
1 mL * 10 mM (in DMSO)	In stock	$ 48.00

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Purity: 98%

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Description	6-Aminonicotinamide (6-AN) is a well-established inhibitor of the NADP+-dependent enzyme.
In vitro	The EC50 of 6-AN on the inhibition of surface antigen (HBsAg) is approximately 23.89 μM in HepAD38 cells with a sensitive index (SI) > 20[1]
In vivo	6-AN decreases HBsAg production but not blocks its secretion[1].
Cell Research	The cytotoxic effects of 6-AN on various cells were assessed by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay .?Cells were seeded into 96-well?plates and incubated with different concentrations (multiple proportion dilution from 500?μM to 0.98?μM) of 6-AN for 72 h. Next, 40?ug/ml MTT were added into cell culture media and incubated for 4 h under the condition of protection from light.?After that, dimethyl sulfoxide (DMSO) (Solarbio) was added and incubated overnight in the dark to lyses the cells and solubilize formazan dissolving in media.?Fifty-percent cytotoxicity concentrations (CC50) and Fifty-percent effective concentrations (EC50) were determined with a microplate reader and calculated via non-linear regression using GraphPad Prism 5.0[1].
Animal Research	HBV-transgenic mice (HBV-Tg C57 BL/6),Mice were selected as age (6 to 8 weeks), weight (21±1 g), and possess of basically the same serum HBsAg and HBV DNA levels.?Next, the mice were assigned to four groups of 10 individuals per group at random: negative control (0.9% saline), positive control (0.02 mg/kg Entecavir (ETV)), low concentration test group (6-AN 2.5 mg/kg), high concentration test group (6-AN 5 mg/kg).?Animals received 0.9% saline or 6-AN via intraperitoneal injection and animals received ETV via oral gavage every two days.?The serum samples were collected via orbital blood every four days after injection.?All blood was collected for analysis of serum HBsAg and HBV markers.?6 mice from each group were sacrificed by cervical dislocation at day 20 and the liver samples were used for intrahepatic HBV DNA and RNA determination.?The remaining animals were monitored without treatment up to day 32[1].

Molecular Weight	137.14
Formula	C6H7N3O
CAS No.	329-89-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 27.5 mg/mL (200.52 mM)

References and Literature

1. Ren F, Yang X, Hu ZW,et al.Niacin analogue, 6-Aminonicotinamide, a novel inhibitor of hepatitis B virus replication and HBsAg production[J].EBioMedicine. 2019 Nov;49:232-246. 2. Shawgi, Hago, Almugadam, et al. Influence of 6-aminonicotinamide (6AN) on Leishmania promastigotes evaluated by metabolomics: Beyond the pentose phosphate pathway.[J]. Chemico-biological interactions, 2018.

Citations

1. Li M, Yang J, Ye C, et al. Integrated Metabolomics and Transcriptomics Analyses Reveal Metabolic Landscape in Neuronal Cells during JEV Infection. Virologica Sinica. 2021: 1-12.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Cancer Active Compound Library Human Metabolite Library Bioactive Compound Library NO PAINS Compound Library Anti-Cancer Compound Library Bioactive Compounds Library Max

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Keywords

6-Aminonicotinamide 329-89-5 Others reactions DNA-crosslinking Inhibitor 6Aminonicotinamide ATP redox 6 Aminonicotinamide antimetabolite nicotinamide inhibit depletion inhibitor

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