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12-Oleanene-3,6-diol

Catalog No. TN6586 CAS 41498-79-7

12-Oleanene-3,6-diol has antitumor activity, it can induce apoptosis and has inhibition effect on the proliferation in RKO cell line.

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12-Oleanene-3,6-diol, CAS 41498-79-7

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5 mg	Inquiry	$ 480.00
1 mL * 10 mM (in DMSO)	Inquiry	$ 490.00

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Description	12-Oleanene-3,6-diol has antitumor activity, it can induce apoptosis and has inhibition effect on the proliferation in RKO cell line.
In vitro	To investigate the effects of novel triterpene (12-Oleanene-3,6-diol ) from Celastrus hypoleucus on the proliferation and apoptosis of human colorectal cancer cell line RKO. METHODS AND RESULTS:The inhibitory effect of the novel triterpene on RKO cell proliferation was assayed by MTT dye reduction. The morphology of apoptotic cells was observed with AO/EB double fluorescence staining and HE staining, DNA fragment with electrophoresis on agarose gels, sub-diploid peak and cell cycle with flow cytometer (FCM). Novel triterpene (12-Oleanene-3,6-diol) from C. hypoleucus significantly inhibited proliferation of RKO cells in dose-dependent and time-dependent manner, the IC50 was (12.20 +/- 0.79) microg x mL(-1) at 48 h. Typical apoptotic changes were observed in RKO cells under the fluorescence microscope and the light microscope. DNA ladder was detected on agarose gels at concentrations from 10 microg x mL(-1) to 20 microg x mL(-1) at 48 h. With FCM methods, dose-dependent apoptosis-induced effect was observed in RKO cell line after treatment of triterpene for 48 h, and the apoptotic rates were increased from(2.93 +/- 0.84) % to (50.79 +/- 6.61) % at concentrations from 2.5 microg x mL(-1) to 20 microg x mL(-1). DNA histograms data from FCM analysis showed that the number of cells was obviously reduced during G0-G1 phase and G2-M phase, but not during S phase for RKO cell line after treatment with various concentrations of the triterpene for 48 hours. CONCLUSIONS:Novel triterpene (12-Oleanene-3,6-diol) from C. hypoleucus can induce apoptosis and has inhibition effect on the proliferation in RKO cell line.
Source	Natural Products > Celastraceae > Celastrus

Molecular Weight	442.72
Formula	C30H50O2
CAS No.	41498-79-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

References and Literature

1. Antitumor effects of novel triterpene from Celastrus hypoleucus on human colorectal cancer cell line RKO in vitro. China journal of Chinese materia medica, 2006, 31(17):1450-1453.

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12-Oleanene-3,6-diol 41498-79-7 12Oleanene3,6diol 12 Oleanene 3,6 diol inhibitor inhibit

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