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ζ-Stat trisodium

Catalog No. T40395 CAS 31894-34-5

Synonyms: NSC37044 trisodium, ζ-Stat trisodium

ζ-Stat trisodium (NSC37044 trisodium) is a potent and selective inhibitor of protein kinase C-ζ (PKC-ζ), exhibiting an IC50 of 5 μM. It effectively suppresses the proliferation of melanoma cell lines and promotes apoptosis. Moreover, ζ-Stat trisodium demonstrates notable antitumor activity in vitro.

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ζ-Stat trisodium, CAS 31894-34-5

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Description	ζ-Stat trisodium (NSC37044 trisodium) is a potent and selective inhibitor of protein kinase C-ζ (PKC-ζ), exhibiting an IC50 of 5 μM. It effectively suppresses the proliferation of melanoma cell lines and promotes apoptosis. Moreover, ζ-Stat trisodium demonstrates notable antitumor activity in vitro.
Targets&IC50	APKCζ:5 μM (IC50)
In vitro	ζ-Stat, administered at concentrations ranging from 0.1 to 20 μM, exhibits minimal inhibition (13%) of PKC-ι at the highest concentration (20 μM), but significantly inhibits PKC-ζ (51%) at a lower concentration of 5 μM. In studies conducted over three days, ζ-Stat demonstrates a dose-dependent reduction in cell proliferation in SK-MEL-2 and MeWo cell lines at concentrations between 0.1 and 10 μM. When combined with 5-FU, ζ-Stat, at concentrations of 7 or 10 μM over periods of 24 to 72 hours, significantly reduces the viability of LoVo CRC cells by more than 75%. Additionally, ζ-Stat at a concentration of 5 μM over three days markedly decreases levels of phosphorylated and total PKC-ζ, Bcl-2, and PARP, while increasing Caspase-3 and cleaved-PARP levels in SK-MEL-2 and MeWo cells. However, ζ-Stat shows no significant cytotoxicity at 5 μM over a duration of 1 to 10 hours in MEL-F-NEO, SK-MEL-2, and MeWo cells. Proliferation assays reveal a 47.7% reduction in SK-MEL-2 cells and a 50.6% decrease in MeWo cells at a concentration of 5 μM after three days, with a notable 19.3% inhibition in MEL-F-NEO cells at 10 μM. Western Blot analysis confirms the reduction in phosphorylated and total PKC-ζ levels in SK-MEL-2 and MeWo cells at a concentration of 5 μM.

Synonyms	NSC37044 trisodium, ζ-Stat trisodium
Molecular Weight	453.31
Formula	C10H8Na3O10S3
CAS No.	31894-34-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

References and Literature

1. Ratnayake WS, et, al. Oncogenic PKC-ι activates Vimentin during epithelial-mesenchymal transition in melanoma; a study based on PKC-ι and PKC-ζ specific inhibitors. Cell Adh Migr. 2018; 12(5):447-463. 2. Islam SMA, et, al. Atypical Protein Kinase-C inhibitors exhibit a synergistic effect in facilitating DNA damaging effect of 5-fluorouracil in colorectal cancer cells. Biomed Pharmacother. 2020 Jan; 121:109665.

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Keywords

ζ-Stat trisodium 31894-34-5 NSC37044 NSC 37044 ζStat trisodium NSC37044 trisodium ζ-Stat ζ Stat trisodium NSC-37044 inhibitor inhibit

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