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β-Amyloid (1-42), (rat/mouse) (TFA)

Catalog No. T35499

Synonyms: Amyloid β-peptide (1-42) (human) TFA, 166090-74-0 TFA

β-Amyloid (1-42), (rat/mouse) (TFA) is a peptide fragment consisting of 42 amino acids that can be used to study Alzheimer's disease.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

β-Amyloid (1-42), (rat/mouse) (TFA) Chemical Structure

β-Amyloid (1-42), (rat/mouse) (TFA), CAS N/A

Pack Size	Availability	Price/USD	Quantity
1 mg	In stock	$ 266.00
5 mg	In stock	$ 995.00

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Biological Description

Chemical Properties

Storage & Solubility Information

Description

β-Amyloid (1-42), (rat/mouse) (TFA) is a peptide fragment consisting of 42 amino acids that can be used to study Alzheimer's disease.

In vitro

β-Amyloid Aggregation Guidelines (This protocol provides only a guideline and should be modified according to your specific needs).
Dissolve the solid Aβ peptide in cold hexafluoro-2-propanol (HFIP). Incubate the peptide at room temperature for at least 1 hour to establish monomerization and randomization of the structural formation.Evaporate HFIP to preserve the obtained peptide in a film form at -20 or -80°C.Dissolve the obtained film in anhydrous DMSO (5 mM) and then vortex to dilute it to an appropriate concentration in a buffer (serum-free and phenol red-free medium).Subsequently, age the solution at 4-8°C for 48 hours. Centrifuge the samples at 14,000 g for 10 minutes at 4-8°C; soluble oligomers will be present in the supernatant. Dilute the supernatant 10-200 times for experimental use.The method may vary based on downstream applications.

Synonyms	Amyloid β-peptide (1-42) (human) TFA, 166090-74-0 TFA
Molecular Weight	4532.04

Storage

store at low temperature,keep away from moisture

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 50 mg/mL (11.03 mM), Sonication is recommended.

References and Literature

1. Mozes E, et al. A novel method for the rapid determination of beta-amyloid toxicity on acute hippocampal slices using MTT and LDH assays. Brain Res Bull. 2012 Apr 10;87(6):521-5. 2. Stefania Sabella, et al. Capillary electrophoresis studies on the aggregation process of beta-amyloid 1-42 and 1-40 peptides. Electrophoresis. 2004 Oct;25(18-19):3186-94. 3. Lagunes T, et al. Abeta(1-42) induces abnormal alternative splicing of tau exons 2/3 in NGF-induced PC12 cells. An Acad Bras Cienc. 2014 Dec;86(4):1927-34.

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You can also refer to dose conversion for different animals. More

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

β-Amyloid (1-42), (rat/mouse) (TFA) β Amyloid (1 42), (rat/mouse) (TFA) Amyloid β-peptide (1-42) (human) TFA b-Amyloid , (rat/mouse) β-Amyloid , (rat/mouse) 166090-74-0 TFA βAmyloid (142), (rat/mouse) (TFA) beta-Amyloid , (rat/mouse) inhibitor inhibit

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.