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Y-33075 dihydrochloride

Name: Y-33075 dihydrochloride
Brand: TargetMol
SKU: T13384L
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T13384LCas No. 173897-44-4

Y-33075 dihydrochloride is a selective inhibitor of ROCK with an IC50 of 3.6 nM.

Y-33075 dihydrochloride

🥰Excellent

Purity: 99.89%

Catalog No. T13384LCas No. 173897-44-4

Y-33075 dihydrochloride is a selective inhibitor of ROCK with an IC50 of 3.6 nM.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$30	In Stock	In Stock
5 mg	$64	In Stock	In Stock
10 mg	$109	In Stock	In Stock
25 mg	$213	In Stock	In Stock
50 mg	$379	In Stock	In Stock
100 mg	$567	In Stock	In Stock
200 mg	$813	-	In Stock
1 mL x 10 mM (in DMSO)	$75	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:99.89%

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	Y-33075 dihydrochloride is a selective inhibitor of ROCK with an IC50 of 3.6 nM.
Targets&IC50	CaMK II:810 nM, PKC:420 nM, ROCK:3.6 nM
In vitro	Y-33075 (Y-39983) is a potent inhibitor of ROCK with an IC50 of 3.6 nM and exhibits greater inhibitory effects on PKC and CaMKII compared to Y-27632. The IC50 values for PKC are 9.0 μM for Y-27632 and 0.42 μM for Y-33075, while for CaMKII, the IC50s are 26 μM and 0.81 μM, respectively. The IC50s for Y-27632 and Y-33075 for PKC are 82 and 117 times higher than for ROCK, respectively, and for CaMKII, they are 236 and 225 times higher than for ROCK, respectively [1]. Y-33075 (Y-39983, 10 μM) promotes neurite extension in retinal ganglion cells (RGCs) compared to untreated cells [2], and at 1 μM, it inhibits the histamine-evoked contraction of rabbit ciliary artery segments in Ca2+-free solutions [3].
In vivo	Y-33075 (≥0.01%) significantly lowers intraocular pressure (IOP) at 2 hours after topical administration in rabbits. Y-33075 (0.05%)-treated eyes show significant reduction of IOP between 2 and 7 hours after topical administration in monkeys[1]. Y-33075 (100 μM) increases the regenerating axons of retinal ganglion cells (RGCs) in the eyes of the rats[2].

Chemical Properties

Molecular Weight	353.25
Formula	C₁₆H₁₈Cl₂N₄O
Cas No.	173897-44-4
Smiles	Cl.Cl.C[C@@H](N)c1ccc(cc1)C(=O)Nc1ccnc2[nH]ccc12
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (283.09 mM), Sonication is recommended.

H2O: 50 mg/mL (141.54 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (11.32 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8309 mL	14.1543 mL	28.3086 mL	141.5428 mL
5 mM	0.5662 mL	2.8309 mL	5.6617 mL	28.3086 mL
10 mM	0.2831 mL	1.4154 mL	2.8309 mL	14.1543 mL
20 mM	0.1415 mL	0.7077 mL	1.4154 mL	7.0771 mL
50 mM	0.0566 mL	0.2831 mL	0.5662 mL	2.8309 mL
100 mM	0.0283 mL	0.1415 mL	0.2831 mL	1.4154 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc