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Thiamet G (Synonyms: TMG)

Name: Thiamet G
Brand: TargetMol
SKU: T6056
Price: 37 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No. T6056 Copy Product Info

Purity: 99.98%

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Thiamet G is a potent O-GlcNAcase inhibitor (Ki=20 nM) with selectivity. The role of O-GlcNAcase is to remove O-GlcNAc from the modified protein.

Thiamet G

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🥰Excellent

Catalog No. T6056

Synonyms TMG

Thiamet G is a potent O-GlcNAcase inhibitor (Ki=20 nM) with selectivity. The role of O-GlcNAcase is to remove O-GlcNAc from the modified protein.

Cas No. 1009816-48-1

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Pack Size	Price	USA Stock	Global Stock
2 mg	$37	In Stock	In Stock
5 mg	$57	In Stock	In Stock
10 mg	$94	In Stock	In Stock
25 mg	$198	In Stock	In Stock
50 mg	$361	In Stock	In Stock
100 mg	$537	In Stock	In Stock
500 mg	$1,190	-	In Stock
1 mL x 10 mM (in DMSO)	$57	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.98%

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	Thiamet G is a potent O-GlcNAcase inhibitor (Ki=20 nM) with selectivity. The role of O-GlcNAcase is to remove O-GlcNAc from the modified protein.
Targets&IC50	O-GlcNAcase (OGA, human):20 nM (Ki), O-GlcNAcase (OGA):30 nM (EC50), O-GlcNAcase (OGA):21 nM (Ki)
In vitro	METHODS: PC-12 cells were treated with Thiamet G (1 nM-250 μM), and the level of O-GlcNAcase was detected by Western Blot. After PC-12 cells were treated with Thiamet G (100 mM), the level of O-GlcNAcase was detected by Western Blot. RESULTS: Thiamet G inhibited O-GlcNAcase in PC-12 cells in a dose - and time-dependent manner and reduced the phosphorylation level of tau protein. [1] METHODS: Mesangial cells were treated with Thiamet G (12.5, 25 nM), and the O-GlcNAcylation level and p38 phosphorylation level were detected by Western Blot. RESULTS: Thiamet G significantly enhances p38 phosphorylation in glomerular cell lines by increasing O-GlcNAcylation. [2]
In vivo	METHODS: To study the neuroprotective effect of Thiamet G, Thiamet G (20 mg/kg) was intraperitoneally injected into mice for 3 consecutive days. RESULTS: Thiamet G exhibited neuroprotective effects by inhibiting O-GlcNAcase. [3] METHODS: To study the antiepileptic effect of Thiamet G, Thiamet G (10 mg/kg) was intraperitoneally injected into an epileptic rat model once a day for three consecutive days. RESULTS: Thiamet G significantly reduced the duration of epileptic seizures and the frequency of interictal spikes. [4]
Kinase Assay	All enzymatic assays are performed in triplicate at 37°C using 4-methylumbelliferyl N-acetyl-β-d-glucosaminide dehydrate as substrate. 1 nM of purified OGA is incubated with the compounds for 5 min, and then 0.2 mM of the substrate is added. The liberation of 4-methylumbellifery is monitored by kinetic reading at excitation/emission 355/460 nm using a Tecan M200 plate in a mode of 60 s/cycle and 15 cycles in total.
Cell Research	Jurkat cells are seeded at 6000 cells/well in a 96-well plate, and 12 h later, cells are treated with compounds for the indicated time. Cell viability is determined by XTT assay.
Synonyms	TMG

Chemical Properties

Molecular Weight	248.3
Formula	C₉H₁₆N₂O₄S
Cas No.	1009816-48-1
Smiles	[H][C@]12O[C@H](CO)[C@@H](O)[C@H](O)[C@@]1([H])N=C(NCC)S2
Relative Density.	1.74 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 50 mg/mL (201.37 mM), Sonication is recommended.

DMSO: 100 mg/mL (402.74 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (16.11 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	4.0274 mL	20.1369 mL	40.2739 mL	201.3693 mL
5 mM	0.8055 mL	4.0274 mL	8.0548 mL	40.2739 mL
10 mM	0.4027 mL	2.0137 mL	4.0274 mL	20.1369 mL
20 mM	0.2014 mL	1.0068 mL	2.0137 mL	10.0685 mL
50 mM	0.0805 mL	0.4027 mL	0.8055 mL	4.0274 mL
100 mM	0.0403 mL	0.2014 mL	0.4027 mL	2.0137 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc